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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Status of p16
INK4a
and E-cadherin gene promoter methylation in Moroccan patients with cervical carcinoma
Oncology Research, Volume 18, No. 4, Year 2009
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Description
Aberrant methylation of tumor suppressor gene promoters has been extensively investigated in cervical cancer. Transcriptional silencing, as a main consequence of hypermethylation of CpG islands, is the predominant mechanism of p16INK4a and E-cadherin gene inactivation in malignant epithelial tumors. This study was conducted to evaluate the promoter methylation status of p16INK4a and E-cadherin genes in 22 specimens of cervical carcinomas, four cervical cancer cell lines (HeLa, SiHa, Caski, C33A), and 20 human papillomavirus negative specimens, obtained from normal cervical swabs, using the methylation-specific PCR approach. Hypermethylation of the 5′ CpG island of the p16INK4a and E-cadherin genes were found in 13 (59.1%) and 10 (45.5%) of 22 cervical cancer samples, respectively. Furthermore, our findings did not show any correlation between promoter methylation of p16INK4a and E-cadherin genes and clinicopathological parameters, including HPV infection, phenotypic distribution, and stage of the disease. However, hypermethylation of E-cadherin gene promoter appears to be age related in cervical cancer, whereas the frequency of aberrant methylation of p16INK4a gene promoter is unchanged according to the age of patients. Thus, caution must be made to use these markers in the diagnosis of cervical cancer. However, dietary or pharmaceutical agents that can inhibit these epigenetic events may prevent or delay the development of cervical cancer. Copyright © 2009 Cognizant Comm. Corp.
Authors & Co-Authors
Attaleb, M.
Morocco, Rabat
National Energy Center of Nuclear Science and Technology
Hamadani, Wail El
Morocco, Rabat
National Energy Center of Nuclear Science and Technology
Khyatti, Meriem
Morocco, Casablanca
Institut Pasteur du Maroc
Benbacer, Laïla
Morocco, Rabat
National Energy Center of Nuclear Science and Technology
Benchekroun, Nadia
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Benider, Abdellatif
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Amrani, Mariam
Morocco, Agdal Rabat
National Institute of Oncology, Rabat
El-Mzibri, Mohammed E.
Morocco, Rabat
National Energy Center of Nuclear Science and Technology
Statistics
Citations: 25
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.3727/096504009790217416
ISSN:
09650407
Research Areas
Cancer
Genetics And Genomics