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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Vaccine, Volume 21, No. 11-12, Year 2003
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Description
Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the eukaryotic one, confers protection comparable to that induced by the full-length molecule, but is unstable. Peptides encoding 80 (p67C) and 205 (p67N) amino acid fragments of p67, containing epitopes recognised by sporozoite neutralising monoclonal antibodies, exhibit improved stability in E. coli. Antibodies raised against the central region of p67 (p67M) neutralise sporozoite infectivity in vitro. The p67C peptide induced immunity against ECF in cattle, at a level equivalent to p67635, suggesting that a synthetic peptide vaccine might be achievable. © 2002 Elsevier Science Ltd. All rights reserved.
Authors & Co-Authors
Bishop, Richard Peter
Kenya, Nairobi
International Livestock Research Institute Nairobi
Nene, Vishvanath M.
Kenya, Nairobi
International Livestock Research Institute Nairobi
United States, Rockville
J. Craig Venter Institute
Staeyert, Jan
Kenya, Nairobi
International Livestock Research Institute Nairobi
Belgium, Brussels
Vrije Universiteit Brussel
Rowlands, John
Kenya, Nairobi
International Livestock Research Institute Nairobi
Nyanjui, John K.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Osaso, Julius
Kenya, Nairobi
International Livestock Research Institute Nairobi
Morzaria, Subhash P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Musoke, Anthony Jim Tony
Kenya, Nairobi
International Livestock Research Institute Nairobi
Statistics
Citations: 36
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/S0264-410X(02)00621-7
ISSN:
0264410X