Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Structural, functional, and evolutionary characterization of novel members of the allatostatin receptor family from insects
Biochemical and Biophysical Research Communications, Volume 282, No. 4, Year 2001
Notification
URL copied to clipboard!
Description
By using degenerate primers based on known mammalian somatostatin receptors and the recently identified Drosophila allatostatin receptors (AlstR), we have cloned a novel receptor for the neuropeptide, allatostatin, from the cockroach Periplaneta americana. The receptor exhibits about 60% amino acid identity in the transmembrane regions when compared to the two known AlstRs from Drosophila melanogaster. In addition, two cDNA fragments were obtained from the stick insect Carausius morosus, one of which is similar to Drosophila AlstR, whereas the other is more similar to mammalian somatostatin receptors. Functional expression in Xenopus oocytes shows that the Periplaneta-AlstR exhibits high affinity to endogenous cockroach allatostatin peptides. Studies with synthetic peptides demonstrate that agonistic activity is mediated by the conserved C-terminal pentapeptide YXFGL-amide; in this sequence, amidation of the C-terminus is obligatory to maintain affinity. Thus, our studies provide a molecular basis for understanding the widespread biological activities of the allatostatin peptides. © 2001 Academic Press.
Authors & Co-Authors
Auerswald, Lutz
South Africa, Cape Town
University of Cape Town
Birgül, Necla
Germany, Hamburg
Universität Hamburg
Gáde, Gerd
South Africa, Cape Town
University of Cape Town
Kreienkamp, Hans Jürgen
Germany, Hamburg
Universität Hamburg
Richter, Dietmar
Germany, Hamburg
Universität Hamburg
Statistics
Citations: 57
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1006/bbrc.2001.4659
ISSN:
0006291X
Research Areas
Genetics And Genomics