Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Schistosoma mansoni: Detection of circulating antigens in murine schistosomiasis by antigen-capture sandwich ELISA using a monoclonal antibody
Experimental Parasitology, Volume 71, No. 1, Year 1990
Notification
URL copied to clipboard!
Description
A monoclonal antibody (MAb) 5H11/B1 that reacts with a repeating epitope on an excretory-secretory (E + S) antigen of adult worms of Schistosoma mansoni was used in the detection of circulating antigen (CA) in sera from S. mansoni-infected mice using an antigen-capture sandwich ELISA. Trichloroacetic acid (TCA) pretreatment of sera from mice infected for 8 or 16 weeks precipitated immune complexes and/or dissociated CA and allowed its detection. Sera obtained 8 weeks after infection contained high levels of CA. Upon treatment with praziquantel (100 mg/kg body wt), this level was significantly less within 1 week. A strong correlation was found between the worm count determined by perfusion and the level of antigenemia detected by the 5H11/B1 assay in light and heavy infection (r = 0.80). Based on the results of both TCA pretreatment and sodium periodate treatment, the 5H11/B1 sandwich ELISA assay detects a repeating carbohydrate epitope on an E + S antigen. This system appears to be a sensitive assay for the detection of schistosomal antigenemia in murine schistosomiasis. Studies on the detection of antigenemia in human schistosomiasis using this assay are in progress. © 1990.
Authors & Co-Authors
Barsoum, Ibrahim S.
United States, Nashville
Vanderbilt University School of Medicine
United States
Va Medical Center
Colley, Daniel G.
United States, Nashville
Vanderbilt University School of Medicine
United States
Va Medical Center
Kamal, Karim
United States, Nashville
Vanderbilt University School of Medicine
United States
Va Medical Center
Egypt, Cairo
Us Naval Medical Research Unit Number 3
Statistics
Citations: 28
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1016/0014-4894(90)90013-3
ISSN:
00144894
e-ISSN:
10902449
Research Areas
Infectious Diseases