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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
HIV-1 Subtypes and Recombinants in Northern Tanzania: Distribution of Viral Quasispecies
PLoS ONE, Volume 7, No. 10, Article e47605, Year 2012
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Description
This study analyzed the distribution and prevalence of HIV-1 subtypes, multiplicity of HIV-1 infection, and frequency of inter-subtype recombination among HIV-1-infected female bar and hotel workers in Moshi, Kilimanjaro Region, Tanzania, from 2004 to 2007. The HIV-1 viral sequences spanning the V1-C5 region of HIV-1 env gp120 were analyzed from 50 subjects by single genome amplification and sequencing (SGA/S) technique. A total of 1740 sequences were amplified and sequenced from the HIV-1 proviral DNA template. The median env sequences analyzed per subject per two time points was 38 (IQR 28-50) over one year of HIV infection. In a subset of 14 subjects, a total of 239 sequences were obtained from HIV-1 RNA template at the baseline visit. The most prevalent HIV-1 subtypes were A1 (56%) and C (30%), while HIV-1 subtype D and inter-subtype recombinant viruses were found in 6% and 8% of subjects respectively. Transmission of multiple HIV-1 variants was evident in 27% of the subjects infected with pure HIV-1 subtypes A1, C, or D. The HIV-1 inter-subtype recombinants were found in 8% including HIV-1 C/A, D/A, and complex mosaic recombinants. Multiple viral variants were found in two subjects infected with inter-subtype recombinants. One subject harbored quasispecies of both pure HIV-1 A1 and C/A recombinant. The other subject was infected with two complex mosaic inter-subtype recombinant variants belonging to subtype D. HIV-1 multiple infections and ongoing recombination contribute significantly to the genetic diversity of circulating HIV-1 in Tanzania and have important implications for vaccine design and the development of therapeutic strategies. © 2012 Kiwelu et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s003.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s004.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s005.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3485255/bin/pone.0047605.s006.docx
Authors & Co-Authors
Kiwelu, Ireen Eliah E.
Unknown Affiliation
Novitsky, Vladimir A.
Unknown Affiliation
Margolin, Lauren
Unknown Affiliation
Baca, Jeannie
Unknown Affiliation
Manongi, Rachel N.
Unknown Affiliation
Sam, Noel E.
Unknown Affiliation
Shao, John F.
Unknown Affiliation
McLane, Mary Fran
Unknown Affiliation
Kapiga, Saidi Hussein
Unknown Affiliation
Essex, Max E.
Unknown Affiliation
Statistics
Citations: 21
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0047605
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Tanzania
Participants Gender
Female