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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Diagnosis of Chlamydia trachomatis eye infection in Tanzania by polymerase chain reaction/enzyme immunoassay
The Lancet, Volume 338, No. 8771, Year 1991
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Description
Detection of Chlamydia trachomatis eye infection is largely unsatisfactory by standard laboratory methods. A polymerase chain reaction/enzyme immunoassay (PCR-EIA) that had previously been successful for diagnosis of genital C trachomatisinfection was compared with direct antibody immunofluorescence (DFA) for detection of the organism in conjunctival scrapes. 234 Tanzanian children aged 1-7 years living in a village that had had no previous trachoma control programme were classified clinically as having no sign of trachoma (0) n=97, follicular trachoma (TF) n=100, or intense inflammatory trachoma with or without TF (TI ± TF) n=37. PCR-EIA detected C trachomatis in 24%, 54%, and 95% of subjects, respectively, compared with elementary body (EB) detection by DFA of 1%, 28%, and 60%, respectively. Overall prevalence of chlamydial eye infection was 22% by DFA compared with 48% by PCR-EIA. Of subjects with chlamydial DNA at pretreatment, 103 (92%) had no detectable chlamydial DNA at the end of 4 weeks of ocular tetracycline. The findings show that PCR-EIA is likely to affect trachoma diagnosis and epidemiology because of the increased sensitivity for detection of C trachomatis in all clinical groups; the less stringent requirements for specimen collection and transport make this method suitable for field use. Moreover, the semi-quantitative aspect of PCR-EIA may be useful for monitoring a decrease in chlamydial DNA after treatment. © 1991.
Authors & Co-Authors
Bobo, Linda D.
United States, Baltimore
Johns Hopkins School of Medicine
Viscidi, Raphael P.
United States, Baltimore
Johns Hopkins School of Medicine
Quinn, Thomas Charles
United States, Baltimore
Johns Hopkins School of Medicine
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Vos, Theo K.
United States, Baltimore
Wilmer Eye Institute
Mkocha, Harran A.
Tanzania, Kongwa
Kongwa Trachoma Project
Muñoz, Beatriz E.
United States, Baltimore
Wilmer Eye Institute
Statistics
Citations: 99
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/0140-6736(91)91502-L
ISSN:
01406736
Research Areas
Genetics And Genomics
Health System And Policy
Maternal And Child Health
Sexual And Reproductive Health
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Tanzania