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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Lubricin expression in human osteoarthritic knee meniscus and synovial fluid: A morphological, immunohistochemical and biochemical study
Acta Histochemica, Volume 116, No. 5, Year 2014
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Description
The purpose of this study was to investigate the expression of lubricin, the product of the human PRG4 (proteoglycan 4) gene, in menisci and synovial fluid from normal donors and patients with osteoarthritis (OA), using a combination of histology, immunohistochemistry, ELISA and Western blotting analysis, to provide further insight on the role of this protein in the progression of OA and pathological processes in the meniscus. Lubricin expression was studied in samples from 40 patients and in 9 normal donors after arthroscopic partial meniscectomy. Histological analysis confirmed normal microanatomy and the absence of structural changes in control samples. Menisci derived from OA patients showed evidence of structural alterations, fibrillations and clefts. Immunohistochemical analysis revealed very strong lubricin immunostaining in normal menisci in contrast to weak/moderate staining seen in osteoarthritic menisci. Quantitative ELISA and Western blot analysis confirmed the above results. The findings of this study support the notion that changes in lubricin expression and boundary-lubricating ability of cartilage is followed by the development of OA. This study could provide the biological foundation for the development of novel therapeutic treatments, to be applied before the surgery, for the prevention of post-traumatic cartilage damage. © 2014 Elsevier GmbH.
Authors & Co-Authors
Musumeci, Giuseppe
Italy, Catania
Università Degli Studi Di Catania
Trovato, Francesca M.
Italy, Catania
Università Degli Studi Di Catania
Loreto, Carla
Italy, Catania
Università Degli Studi Di Catania
Leonardi, Rosalia Maria
Italy, Catania
Università Degli Studi Di Catania
Szychlinska, Marta Anna
Italy, Catania
Università Degli Studi Di Catania
Castorina, Sergio
Italy, Catania
Università Degli Studi Di Catania
Italy, Catania
Fondazione Mediterranea G.b. Morgagni
Mobasheri, Ali
United Kingdom, Guildford
University of Surrey
United Kingdom, Nottingham
Nottingham University Hospitals Nhs Trust
Saudi Arabia, Jeddah
King Abdulaziz University
Statistics
Citations: 58
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.acthis.2014.03.011
ISSN:
00651281
e-ISSN:
16180372
Research Areas
Genetics And Genomics
Health System And Policy
Study Approach
Quantitative