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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Characteristics of infections caused by extended-spectrum β-lactamase-producing Escherichia coli from community hospitals in South Africa
Diagnostic Microbiology and Infectious Disease, Volume 69, No. 4, Year 2011
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Description
A study was designed to characterize 22 nonrepeat extended-spectrum β-lactamase (ESBL)-producing Escherichia coli clinical isolates recovered from specimens originating from doctor's consultation rooms and several private and a state hospital in the Cape Town metropolitan area during 2008-2009. Characterization was done by using isoelectric focusing, PCR, sequencing of blaCTX-M, blaTEM, blaSHV, and blaOXA as well as PCR for plasmid-mediated quinolone resistance determinants, ST131, phylogenetic groups, and plasmid replicon typing. Genetic relatedness was determined with pulsed-field gel electrophoresis using XbaI and multilocus sequencing typing. The majority of patients (17/22 [77%]) presented with urinary tract infections (UTIs) originating from the hospital setting. Thirteen (59%) of the isolates produced CTX-M-15, 7 produced CTX-M-14, and 1 isolate each produced CTX-M-3 and SHV-2, respectively. Sixteen (73%) isolates were nonsusceptible to ciprofloxacin and 8 (36%) were positive for aac(6')-Ib-cr. Overall, 10/22 (45%) of ESBL producers belonged to clonal complex ST131 that produced CTX-M-15 or CTX-M-14. Molecular characteristics of ST131 showed that this clone belonged to phylogenetic group B2. Our study illustrated that clonal complex ST131 isolates producing CTX-M-15 and CTX-M-14 had emerged as an important cause of UTIs due to ESBL-producing E. coli in the Cape Town area. This is the first report to identify ST131 in ESBL-producing E. coli from Southern Africa. © 2011 Elsevier Inc.
Authors & Co-Authors
Peirano, Gisele
Canada, Calgary
Calgary Laboratory Services
Van Greune, C. H.J.
South Africa, Cape Town
Ampath Laboratories
Pitout, Johann D.D.
Canada, Calgary
Calgary Laboratory Services
Canada, Calgary
University of Calgary
Statistics
Citations: 63
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1016/j.diagmicrobio.2010.11.011
ISSN:
07328893
Research Areas
Genetics And Genomics
Health System And Policy
Study Locations
South Africa