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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Discovery and mapping of a new expressed sequence tag-single nucleotide polymorphism and simple sequence repeat panel for large-scale genetic studies and breeding of Theobroma cacao L.
DNA Research, Volume 19, No. 1, Year 2012
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Description
Theobroma cacao is an economically important tree of several tropical countries. Its genetic improvement is essential to provide protection against major diseases and improve chocolate quality. We discovered and mapped new expressed sequence tag-single nucleotide polymorphism (EST-SNP) and simple sequence repeat (SSR) markers and constructed a high-density genetic map. By screening 149 650 ESTs, 5246 SNPs were detected in silico, of which 1536 corresponded to genes with a putative function, while 851 had a clear polymorphic pattern across a collection of genetic resources. In addition, 409 new SSR markers were detected on the Criollo genome. Lastly, 681 new EST-SNPs and 163 new SSRs were added to the pre-existing 418 co-dominant markers to construct a large consensus genetic map. This high-density map and the set of new genetic markers identified in this study are a milestone in cocoa genomics and for marker-assisted breeding. The data are available at http://tropgenedb. cirad.fr. © 2011 The Author.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_19_1_23__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_dsr039_dsr039supp_table1.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_dsr039_dsr039supp_table2.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_dsr039_dsr039supp_table3.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_dsr039_dsr039supp_table4.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3276266/bin/supp_dsr039_dsr039supp_table5.xls
Authors & Co-Authors
Allegre, Mathilde
France, Paris
Cirad
Argout, Xavier
France, Paris
Cirad
Boccara, Michel
France, Paris
Cirad
Trinidad and Tobago, St Augustine
The University of the West Indies, St. Augustine Campus
Fouet, Olivier
France, Paris
Cirad
Roguet, Yolande
France, Paris
Cirad
Bérard, Aurélie
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Thévenin, Jean Marc
France, Paris
Cirad
Chauveau, Aurélie
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Rivallan, Ronan
France, Paris
Cirad
Clément, Didier Pierre Louis
France, Paris
Cirad
Brazil, Itabuna
Comissão Executiva de Planejamento da Lavoura Cacaueira Ceplac
Courtois, Brigitte
France, Paris
Cirad
Peres Gramacho, Karina
Brazil, Itabuna
Comissão Executiva de Planejamento da Lavoura Cacaueira Ceplac
Boland, Anne
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Tahi, Mathias Gnion
Cote D'ivoire, Abidjan
Centre National de Recherche Agronomique Abidjan
Umaharan, Pathmanathan
Trinidad and Tobago, St Augustine
The University of the West Indies, St. Augustine Campus
Brunel, Dominique
France, Versailles
Centre de Recherche Île-de-france - Versailles-grignon
Lanaud, Claire
France, Paris
Cirad
Statistics
Citations: 51
Authors: 17
Affiliations: 5
Identifiers
Doi:
10.1093/dnares/dsr039
ISSN:
13402838
e-ISSN:
17561663
Research Areas
Cancer
Genetics And Genomics