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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Emergence of bluetongue serotypes in Europe, Part 2: The occurrence of a BTV-11 strain in Belgium
Transboundary and Emerging Diseases, Volume 56, No. 9-10, Year 2009
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Description
An EDTA-blood sample from a cow without clinical signs, which gave early birth to a newborn calf that died soon after delivery, was shown to be positive for bluetongue virus (BTV)-RNA using a group-specific real-time RT-PCR (RT-qPCR). In-house serotype-specific RT-qPCR assays for bluetongue virus serotype 1 (BTV-1), -6 and -8 all gave negative results. Subsequent assays were carried out using conventional (gel-based) RT-PCR primers for all 25 BTV serotypes and only two primer sets, both specific for BTV-11, gave bands of the expected size. The cDNAs generated were sequenced and comparisons of the genome segment 2 sequence with that of the modified 'live' vaccine strain of BTV-11 from South Africa showed 100% identity. A survey of all ruminants in a 1-km area around the first positive farm using a BTV-11 serotype-specific RT-qPCR revealed five other holdings with in total nine BTV-11 positive animals. A cross-sectional monitoring of dairy cattle in Belgium showed an overall prevalence of 3.8% on herd level and 0.2% on animal level. A BTV-11 has been introduced into the Belgian cattle herd during the 2008 vector season. The source of the infection and the way by which the virus was introduced are unknown. © 2009 Blackwell Verlag GmbH.
Authors & Co-Authors
de Clercq, Kris E.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Mertens, Peter Paul Clement
United Kingdom, Surrey
The Pirbright Institute
de Leeuw, Ilse
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Oura, Christopher A.L.
United Kingdom, Surrey
The Pirbright Institute
Houdart, P.
Belgium, Brussels
Federal Agency for the Safety of the Food Chain
Potgieter, Abraham Christiaan
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Maan, Sushila
United Kingdom, Surrey
The Pirbright Institute
Hooyberghs, J.
Belgium, Brussels
Federal Agency for the Safety of the Food Chain
Batten, Carrie Anne
United Kingdom, Surrey
The Pirbright Institute
Vandemeulebroucke, Elise L.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Wright, I. M.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Maan, Narender Singh
United Kingdom, Surrey
The Pirbright Institute
Riocreux, F.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Sanders, A.
United Kingdom, Surrey
The Pirbright Institute
Vanderstede, Y.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Nomikou, Kyriaki
United Kingdom, Surrey
The Pirbright Institute
Raemaekers, M.
Belgium, Brussels
Federal Agency for the Safety of the Food Chain
Bin-Tarif, Abdelghani
United Kingdom, Surrey
The Pirbright Institute
Shaw, Andrew E.
United Kingdom, Surrey
The Pirbright Institute
Henstock, Mark R.
United Kingdom, Surrey
The Pirbright Institute
Bréard, Emmanuel
France, Maisons-alfort
Ecole Nationale Vétérinaire D'alfort
Dubois, E.
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Gastaldi-Thiéry, C.
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Zientara, S.
France, Maisons-alfort
Ecole Nationale Vétérinaire D'alfort
Verheyden, B.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Vandenbussche, Frank E.
Belgium, Brussels
Veterinary and Agrochemical Research Centre
Statistics
Citations: 65
Authors: 26
Affiliations: 6
Identifiers
Doi:
10.1111/j.1865-1682.2009.01092.x
ISSN:
18651674
e-ISSN:
18651682
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
South Africa