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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants
Transgenic Research, Volume 21, No. 6, Year 2012
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Description
Rice accumulates high level of arsenic (As) in its edible parts and thus plays an important role in the transfer of As into the food chain. However, the mechanisms of As uptake and its detoxification in rice are not well understood. Recently, members of the Nodulin 26-like intrinsic protein (NIP) subfamily of plant aquaporins were shown to transport arsenite in rice and Arabidopsis. Here we report that members of the rice plasma membrane intrinsic protein (PIP) subfamily are also involved in As tolerance and transport. Based on the homology search with the mammalian AQP9 and yeast Fps1 arsenite transporters, we identified and cloned five rice PIP gene subfamily members. qRT-PCR analysis of PIPs in rice root and shoot tissues revealed a significant down regulation of transcripts encoding OsPIP1;2, OsPIP1;3, OsPIP2;4, OsPIP2;6, and OsPIP2;7 in response to arsenite treatment. Heterologous expression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Xenopus laevis oocytes significantly increased the uptake of arsenite. Overexpression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Arabidopsis yielded enhanced arsenite tolerance and higher biomass accumulation. Further, these transgenic plants showed no significant accumulation of As in shoot and root tissues in long term uptake assays. Whereas, short duration exposure to arsenite caused both active influx and efflux of As in the roots. The data suggests a bidirectional arsenite permeability of rice PIPs in plants. These rice PIPs genes will be highly useful for engineering important food and biofuel crops for enhanced crop productivity on contaminated soils without increasing the accumulation of toxic As in the biomass or edible tissues. © 2012 Springer Science+Business Media B.V.
Authors & Co-Authors
Mosa, Kareem A.
United States, Amherst
University of Massachusetts Amherst
Egypt, Cairo
Faculty of Agriculture
Kumar, Kundan K.Phani
United States, Amherst
University of Massachusetts Amherst
Chhikara, Sudesh
United States, Amherst
University of Massachusetts Amherst
Mcdermott, Joseph
United States, Rochester
Oakland University
Liu, Zijuan
United States, Rochester
Oakland University
Musante, Craig L.
United States, New Haven
Connecticut Agricultural Experiment Station
White, Jason C.
United States, New Haven
Connecticut Agricultural Experiment Station
Dhankher, Om Parkash
United States, Amherst
University of Massachusetts Amherst
Statistics
Citations: 168
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1007/s11248-012-9600-8
ISSN:
09628819
Research Areas
Environmental
Food Security
Genetics And Genomics