Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis
Journal of Antimicrobial Chemotherapy, Volume 69, No. 2, Article dkt353, Year 2014
Notification
URL copied to clipboard!
Description
Objectives: To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples. Methods: The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin. Results: Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%. Conclusions: Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis. © The Author 2013. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Martin, Anandi
Belgium, Ghent
Ghent University, Laboratory of Microbiology
Imperiale, Belen
Argentina, Vicente Lopez
Hospital Zonal de Agudos y Crónicos Dr. Antonio A. Cetrángolo
Ravolonandriana, Pascaline
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Coban, Ahmet Yilmaz
Turkey, Samsun
Ondokuz Mayis Üniversitesi
Akgunes, Alper
Italy
Chest Diseases Hospital
Ikram, Aamer
Pakistan, Multan
Combined Military Hospital, Multan
Satti, Luqman
Pakistan, Multan
Combined Military Hospital, Multan
Odoun, Mathieu
Benin, Cotonou
Laboratoire de Référence Des Mycobactéries
Pandey, Pooja
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Mishra, Manvi
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Affolabi, Dissou
Benin, Cotonou
Laboratoire de Référence Des Mycobactéries
Singh, Urvashi
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Rasolofo-Razanamparany, Voahangy
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Morcillo, Nora Susana
Argentina, Vicente Lopez
Hospital Zonal de Agudos y Crónicos Dr. Antonio A. Cetrángolo
Vandamme, Peter A.
Belgium, Ghent
Ghent University, Laboratory of Microbiology
Palomino, Juan Carlos
Belgium, Ghent
Ghent University, Laboratory of Microbiology
Statistics
Citations: 17
Authors: 16
Affiliations: 8
Identifiers
Doi:
10.1093/jac/dkt353
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Study Design
Cohort Study