Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Tyrosinase inhibition by extracts and constituents of Sideroxylon inerme L. stem bark, used in South Africa for skin lightening
Journal of Ethnopharmacology, Volume 119, No. 3, Year 2008
Notification
URL copied to clipboard!
Description
Objective: To investigate the stem bark of Sideroxylon inerme L. and its compounds for tyrosinase-inhibition activity and to evaluate the mechanism involved of the most potent compounds in tyrosinase inhibition. Materials and methods: Three different extracts (acetone, methanol and dichloromethane) of Sideroxylon inerme L. were evaluated for their inhibitory effect in vitro on the monophenolase and diphenolase activated forms of tyrosinase, using a colorimetric procedure. This test was used for bioactivity-guided isolation of two active compounds using column chromatography and TLC. Active extracts were also investigated for their inhibitory effect on melanogenesis in cultured B16 melanoma cells. Antioxidant activities of the methanolic extract of Sideroxylon inerme and purified compounds were investigated using the 1,2-diphenyl-2-picrylhydrazyl (DPPH) antioxidant assay. The inhibition of tyrosinase activity relative to the inhibition of its activity at the transcriptional level was also studied by determination of the degree of expression of mRNAs for this gene by using extract of Sideroxylon inerme-treated cells (B16F10) and semi-quantitative RT-PCR. Results: Methanolic and acetonic extracts of the stem bark of Sideroxylon inerme showed significant inhibition of monophenolase activity (IC50 values of 63 μg/ml and 82 μg/ml, respectively). The methanolic extract also exhibited 37% reduction of melanin content at 6.2 μg/ml in melanocytes without being significantly toxic to the cells. Examination for inhibition of monophenoloxidase in situ on TLC, followed by column chromatographic purification of the stem bark extract of Sideroxylon inerme, resulted in the isolation of two active compounds, epigallocatechin gallate and procyanidin B1, with IC50 values against monophenolase of 30 μg/ml and >200 μg/ml, respectively. Epigallocatechin gallate exhibited a greater anti-tyrosinase activity than arbutin. Sideroxylon inerme bark extracts, epigallocatechin gallate and procyanidin B1 exhibited antioxidant DPPH radical scavenging activities with EC50 values of 1.54 μg/ml, 1.33 μg/ml and 1.68 μg/ml, respectively and were not particularly cytotoxic. During mechanism studies it was evident that at the transcription level, Sideroxylon inerme (25 μg/ml) was acting as a potent tyrosinase inhibitor compared to controls (untreated cells and kojic acid). Conclusion: The bark extract of Sideroxylon inerme and the two isolated compounds warrant further investigation in clinical studies to be considered as skin-depigmenting agents. © 2008 Elsevier Ireland Ltd. All rights reserved.
Authors & Co-Authors
Momtaz, Saeideh
South Africa, Pretoria
University of Pretoria
Mapunya, B. M.
South Africa, Pretoria
University of Pretoria
Houghton, Peter John
United Kingdom, London
King's College London
Edgerly, C.
United Kingdom, London
King's College London
Hussein, Ahmed Abdel Fattah
South Africa, Pretoria
University of Pretoria
Naidoo, Sanushka
South Africa, Pretoria
University of Pretoria
Lall, Namrita
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 169
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.jep.2008.06.006
ISSN:
03788741
Research Areas
Environmental
Genetics And Genomics
Study Approach
Quantitative
Study Locations
South Africa