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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Transcribed var genes associated with placental malaria in Malawian women
Infection and Immunity, Volume 74, No. 8, Year 2006
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Description
Determining the diversity of PfEMP1 sequences expressed by Plasmodium falciparum-infected erythrocytes isolated from placentas is important for attempts to develop a pregnancy-specific malaria vaccine. The DBLγ and var2csa DBL3x domains of PfEMP1 molecules are believed to mediate placental sequestration of infected erythrocytes, so the sequences encoding these domains were amplified from the cDNAs of placental parasites by using degenerate oligonucleotides. The levels of specific var cDNAs were then determined by quantitative reverse transcription-PCR. Homologues of var2csa DBL3x were the predominant sequences amplified from the cDNAs of most placental but not most children's parasites. There was 56% identity between all placental var2csa sequences. Many different DBLγ domains were amplified from the cDNAs of placental and children's isolates, var2csa transcripts were the most abundant var transcripts of those tested in 11 of 12 placental isolates and 1 of 6 children's isolates. Gravidity did not affect the levels of var2csa transcripts. We concluded that placental malaria is frequently associated with transcription of var2csa but that other var genes are also expressed, and parasites expressing high levels of var2csa are not restricted to pregnant women. The diversity of var2csa sequences may be important for understanding immunity and for the development of vaccines for malaria during pregnancy. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1539630/bin/iai_74_8_4875__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC1539630/bin/iai_74_8_4875__supplfig1.doc
Authors & Co-Authors
Duffy, Michael F.
Australia, Melbourne
University of Melbourne
Caragounis, Aphrodite
Australia, Melbourne
University of Melbourne
Noviyanti, Rintis
Indonesia, Jakarta
Eijkman Institute for Molecular Biology
Kyriacou, Helen M.
United Kingdom, Edinburgh
The University of Edinburgh
Choong, Ee Ken
Australia, Melbourne
University of Melbourne
Boysen, Katja E.
Australia, Melbourne
University of Melbourne
Healer, Julie
Australia, Melbourne
University of Melbourne
Australia, Melbourne
Walter and Eliza Hall Institute of Medical Research
Rowe, Jane Alexandra
United Kingdom, Edinburgh
The University of Edinburgh
Molyneux, Malcolm Edward
Malawi, Zomba
University of Malawi
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Brown, Graham V.
Australia, Melbourne
University of Melbourne
Rogerson, Stephen J.
Australia, Melbourne
University of Melbourne
Statistics
Citations: 101
Authors: 11
Affiliations: 6
Identifiers
Doi:
10.1128/IAI.01978-05
ISSN:
00199567
Research Areas
Infectious Diseases
Maternal And Child Health
Sexual And Reproductive Health
Study Approach
Quantitative
Participants Gender
Female