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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cancer/Testis Antigen 55 is required for cancer cell proliferation and mitochondrial DNA maintenance
Mitochondrion, Volume 64, Year 2022
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Description
Cancer/Testis Antigens (CTAs) represent a group of proteins whose expression under physiological conditions is restricted to testis but activated in many human cancers. Also, it was observed that co-expression of multiple CTAs worsens the patient prognosis. Five CTAs were reported acting in mitochondria and we recently reported 147 transcripts encoded by 67 CTAs encoding for proteins potentially targeted to mitochondria. Among them, we identified the two isoforms encoded by CT55 for whom the function is poorly understood. First, we found that patients with tumors expressing wild-type CT55 are associated with poor survival. Moreover, CT55 silencing decreases dramatically cell proliferation. Second, to investigate the role of CT55 on mitochondria, we first show that CT55 is localized to both mitochondria and endoplasmic reticulum (ER) due to the presence of an ambiguous N-terminal targeting signal. Then, we show that CT55 silencing decreases mtDNA copy number and delays mtDNA recovery after an acute depletion. Moreover, demethylation of CT55 promotor increases its expression, which in turn increases mtDNA copy number. Finally, we measured the mtDNA copy number in NCI-60 cell lines and screened for genes whose expression is strongly correlated to mtDNA amount. We identified CT55 as the second highest correlated hit. Also, we show that compared to siRNA scrambled control (siCtrl) treatment, CT55 specific siRNA (siCT55) treatment down-regulates aerobic respiration, indicating that CT55 sustains mitochondrial respiration. Altogether, these data show for first time that CT55 acts on mtDNA copy number, modulates mitochondrial activity to sustain cancer cell proliferation. © 2022
Authors & Co-Authors
Goudenège, David
France, Paris
Inserm
France, Angers
Chu Angers
Guéguen, Naïg
France, Paris
Inserm
France, Angers
Chu Angers
Desquiret-Dumas, Valérie
France, Paris
Inserm
France, Angers
Chu Angers
Chabrun, Floris
France, Paris
Inserm
France, Angers
Chu Angers
Chevrollier, Arnaud
France, Paris
Inserm
Charif, Majida
France, Paris
Inserm
Baris, Olivier Richard
France, Paris
Inserm
Pommier, Yves G.
United States, Bethesda
National Institutes of Health Nih
Lenaers, Guy
France, Paris
Inserm
Khiati, Salim
France, Paris
Inserm
Statistics
Citations: 2
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.mito.2022.02.005
ISSN:
15677249
Research Areas
Cancer
Genetics And Genomics
Health System And Policy