Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Characterization of a repetitive DNA probe for Babesia bigemina
Veterinary Parasitology, Volume 36, No. 1-2, Year 1990
Notification
URL copied to clipboard!
Description
A plasmid (p16) containing a Babesia bigemina DNA insert was selected and labeled with 32P. This probe was evaluated for specificity and sensitivity by dot blot hybridization. The probe was specific and hybridized with only Babesia bigemina DNA, and not DNA from Babesia bovis, bovine leukocyte, Trypanosoma brucei or Anaplasma marginale. The DNA probe detected as little as 10 pg of Babesia bigemina DNA. The probe hybridized with Babesia bigemina isolates from Mexico, the Caribbean region and Kenya. Genomic Babesia bigemina DNA of a Kenyan isolate was digested with restriction endonucleases, and the fragments were separated by gel electrophoresis and Southern blotted. The filter was hybridized with labeled p16 and each endonuclease digestion produced at least 16 resolvable DNA fragments. The inserted Babesia bigemina DNA was approximately 6.3 kb in size. A partial restriction map was constructed. A simple whole blood dot blot procedure was utilized to evaluate the sensitivity of the DNA probe. This probe would detect as few as 150 Babesia bigemina infected erythrocytes contained in a 1-μl sample. The DNA probe has the potential to be a very sensitive and specific diagnostic tool. © 1990.
Authors & Co-Authors
Buening, Gerald M.
United States, Columbia
University of Missouri
Barbet, Anthony F.
United States, Gainesville
University of Florida
Myler, Peter John
United States, Seattle
Seattle Biomedical Research Institute
Mahan, Suman M.
United States, Gainesville
University of Florida
Nene, Vishvanath M.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
McGuire, Travis C.
United States, Pullman
Washington State University Pullman
Statistics
Citations: 24
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1016/0304-4017(90)90089-T
ISSN:
03044017
Research Areas
Genetics And Genomics
Study Locations
Kenya