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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Sequence analysis of the dimerization initiation site of concordant and discordant viral variants superinfecting HIV type 1 patients
AIDS Research and Human Retroviruses, Volume 27, No. 11, Year 2011
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Description
For HIV recombination to occur, the RNAs from two infecting strains within a cell must dimerize at the dimerization initiation site (DIS). We examined the sequence identity at the DIS (697-731bp, Hxb2 numbering engine) in patients superinfected with concordant HIV-1 strains and compared them to those with discordant strains. Viral RNA in sequential plasma from four subjects superinfected with subtype-discordant and two subjects superinfected with subtype-concordant HIV-1 strains was extracted, amplified (5′ LTR-early gag: 526-1200bp, Hxb2 numbering engine), sequenced, and analyzed to determine their compatibility for dimerization in vivo. The concordant viruses infecting the two subjects exhibited identical sequences in the 35-bp-long DIS region while sequences from the discordant viruses revealed single nucleotide changes that were located in the DIS loop (715bp), its flanking nucleotides (710bp and 717bp), and the DIS stem (719bp). Evidence from in vitro experiments demonstrates that these in vivo changes identified can abolish dimerization and reduce recombination frequency. Therefore, these results revealing differences in the DIS of discordant strains versus the similarity noted for the concordant strains may contribute to the differences in the frequency of recombination in patients superinfected with such HIV-1 variants. © 2011, Mary Ann Liebert, Inc.
Authors & Co-Authors
Mayr, Luzia M.
United States, New York
Nyu Grossman School of Medicine
Powell, Rebecca L.R.
United States, New York
Nyu Grossman School of Medicine
Kinge, Thompson N.
Cameroon, Yaounde
Ministry of Public Health Cameroon
Nyambi, Phillipe N.
United States, New York
Nyu Grossman School of Medicine
United States, New York
Manhattan Veterans Affairs Harbor Healthcare Systems
Statistics
Citations: 4
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1089/aid.2011.0010
ISSN:
08892229
e-ISSN:
19318405
Research Areas
Infectious Diseases