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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus
BMC Evolutionary Biology, Volume 12, No. 1, Article 252, Year 2012
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Description
Background: Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). Results: Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G → T: C → A. Conclusions: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state. © 2012 Monjane et al.; licensee BioMed Central Ltd.
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Authors & Co-Authors
Monjane, Adérito Luis
South Africa, Cape Town
University of Cape Town
Pande, Daniel
Kenya, Maseno
Maseno University
Lakay, Francisco M.
South Africa, Cape Town
University of Cape Town
Shepherd, Dionne Natalie
South Africa, Cape Town
University of Cape Town
van der Walt, Eric
South Africa, Cape Town
Kapa Biosystems
Lefeuvre, Pierre F.
France, Saint-denis
Université de la Réunion
Lett, Jean Michel
France, Saint-denis
Université de la Réunion
Varsani, Arvind
New Zealand, Christchurch
University of Canterbury
South Africa, Cape Town
University of Cape Town
Rybicki, Edward P.
South Africa, Cape Town
University of Cape Town
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
South Africa, Cape Town
Centre for High Performance Computing, Cape Town
Statistics
Citations: 10
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1186/1471-2148-12-252
e-ISSN:
14712148
Research Areas
Cancer
Genetics And Genomics