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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species
Plant Disease, Volume 97, No. 6, Year 2013
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Description
Three Monosporascus eutypoides-like isolates recovered from cucurbit plants with symptoms of Monosporascus root rot and vine decline in Tunisia were compared to 28 isolates of M. cannonballus from 12 countries for phenotypic, genomic, and pathogenicity characteristics. Morphologically, M. cannonballus and M. eutypoides-like cultures were similar, each producing fertile perithecia in culture containing globose, smooth, dark brown to black ascospores. Nevertheless, all M. cannonballus isolates had one ascospore per ascus, while M. eutypoides- like isolates had mainly two to three ascospores per ascus (rarely one). The employment of the internal transcribed spacer (ITS) of nuclear ribosomal DNA, the elongation factor 1-α (EF-1α), and the β-tubulin (β-tub) gene sequence diversity analyses and the resulting phylogenies identified a level of polymorphism that enabled separation of M. cannonballus and M. eutypoides-like isolates. All isolates of M. cannonballus had identical EF-1α and β-tub sequences irrespective of very diverse geographic origins, which were different from the EF-1α and β-tub sequences of the M. eutypoides-like isolates (96 and 97% similarity, respectively). Similar results were obtained for the ITS region of rDNA. In addition, of three M. eutypoides-like isolates tested for pathogenicity, all three were pathogenic on watermelon, two were pathogenic on muskmelon, but only one was pathogenic on cucumber. The results demonstrate that the M. eutypoides-like isolates belong to the species M. eutypoides, and that M. cannonballus and M. eutypoides are distinct species. © 2013 The American Phytopathological Society.
Authors & Co-Authors
Ben Salem, Ibtissem
Tunisia, Sousse
Institut Supérieur Agronomique de Chott Mériem
Correia, K. C.
Brazil, Recife
Universidade Federal Rural de Pernambuco
Boughalleb-M’Hamdi, Naïma
Tunisia, Sousse
Institut Supérieur Agronomique de Chott Mériem
Michereff, Sami Jorge
Brazil, Recife
Universidade Federal Rural de Pernambuco
León, Maela
Spain, Valencia
Universitat Politècnica de València
Abad-Campos, Paloma
Spain, Valencia
Universitat Politècnica de València
García-Jiménez, José
Spain, Valencia
Universitat Politècnica de València
Armengol, J.
Spain, Valencia
Universitat Politècnica de València
Statistics
Citations: 23
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1094/PDIS-05-12-0464-RE
ISSN:
01912917
Research Areas
Cancer
Genetics And Genomics
Study Locations
Tunisia