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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Molecular detection of GES-2 extended spectrum β-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa
International Journal of Antimicrobial Agents, Volume 24, No. 1, Year 2004
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Description
Screening for and detection of the novel extended spectrum β-lactamase (ESBL), GES-2 produced by Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371 bp segment of blaGES-2. This should lead to more cost-effective DNA sequencing and sequence interpretation in the laboratory. © 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Authors & Co-Authors
Weldhagen, Gerhard F.
South Africa, Pretoria
Faculty of Health Sciences
South Africa, Johannesburg
National Health Laboratory Service
Prinsloo, Andrea
South Africa, Johannesburg
National Health Laboratory Service
Statistics
Citations: 36
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijantimicag.2003.12.012
ISSN:
09248579
Research Areas
Cancer
Genetics And Genomics
Study Locations
South Africa