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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification
Journal of Virological Methods, Volume 141, No. 1, Year 2007
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Description
Ebola virus (EBOV) causes severe hemorrhagic fever in humans and nonhuman primates with high mortality rates. Rapid identification of the virus is required to prevent spread of the infection. In this study, we developed and evaluated a one-step simple reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay for the rapid detection of Zaire ebolavirus (ZEBOV), the most virulent species of EBOV, targeting the trailer region of the viral genome. The assay could detect 20 copies of the artificial ZEBOV RNA in 26 min with a real time-monitoring detection, and also detect 10-3 FFU of the cell-culture propagated viruses. The reaction time needed to detect 104 FFU of ZEBOV was only 20 min. In addition, the assay was highly specific for ZEBOV. The RT-LAMP assay developed in this study is rapid, simple, highly specific, and sensitive for the detection of ZEBOV, and so may be an effective diagnostic tool. Furthermore, as this technique does not require sophisticated instrumentation, it seems very suitable for diagnosis in the field or laboratories in Ebola outbreak areas such as Central Africa. © 2006 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Kurosaki, Yohei
Japan, Kashiwa
National Research Institute of Police Science
Japan, Kawaguchi
Japan Science and Technology Agency
Japan, Sapporo
Hokkaido University
Takada, Ayato
Japan, Sapporo
Hokkaido University
Ebihara, Hideki
Japan, Tokyo
The University of Tokyo
Grolla, Allen
Canada, Ottawa
Public Health Agency of Canada
Feldmann, Heinz
Canada, Ottawa
Public Health Agency of Canada
Canada, Winnipeg
University of Manitoba
Kawaoka, Yoshihiro K.
Japan, Tokyo
The University of Tokyo
United States, Madison
University of Wisconsin-madison
Yasuda, Jiro
Japan, Kashiwa
National Research Institute of Police Science
Japan, Kawaguchi
Japan Science and Technology Agency
Statistics
Citations: 84
Authors: 7
Affiliations: 7
Identifiers
Doi:
10.1016/j.jviromet.2006.11.031
ISSN:
01660934
Research Areas
Infectious Diseases