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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities
Molecular Microbiology, Volume 90, No. 2, Year 2013
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Description
Summary: Saccharomyces cerevisiaeHal3 and Vhs3 are moonlighting proteins, forming an atypical heterotrimeric decarboxylase (PPCDC) required for CoA biosynthesis, and regulating cation homeostasis by inhibition of the Ppz1 phosphatase. The Schizosaccharomyces pombeORF SPAC15E1.04 (renamed as Sphal3) encodes a protein whose amino-terminal half is similar to Sc Hal3 whereas its carboxyl-terminal half is related to thymidylate synthase (TS). We show that SpHal3 and/or its N-terminal domain retain the ability to bind to and modestly inhibit in vitro S.cerevisiaePpz1 as well as its S.pombe homolog Pzh1, and also exhibit PPCDC activity in vitro and provide PPCDC function in vivo, indicating that SpHal3 is a monogenic PPCDC in fission yeast. Whereas the Sp Hal3 N-terminal domain partially mimics Sc Hal3 functions, the entire protein and its carboxyl-terminal domain rescue the S.cerevisiaecdc21 mutant, thus proving TS function. Additionally, we show that the 70kDa Sp Hal3 protein is not proteolytically processed under diverse forms of stress and that, as predicted, Sphal3 is an essential gene. Therefore, Sphal3 represents a fusion event that joined three different functional activities in the same gene. The possible advantage derived from this surprising combination of essential proteins is discussed. © 2013 John Wiley & Sons Ltd.
Authors & Co-Authors
Molero, Cristina
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Petrényi, Katalin
Hungary, Debrecen
Debreceni Egyetem
González, Asier
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Switzerland, Basel
Biozentrum, Universität Basel
Carmona, Mercè A.
Spain, Barcelona
Universitat Pompeu Fabra Barcelona
Gelis, Samuel
Spain, Cordoba
Universidad de Córdoba
Abrie, J. Albert
South Africa, Stellenbosch
Stellenbosch University
South Africa, Cape Town
University of Cape Town
Strauss, Erick
South Africa, Stellenbosch
Stellenbosch University
Ramos, José J.A.
Spain, Cordoba
Universidad de Córdoba
Dombrádi, Viktor K.
Hungary, Debrecen
Debreceni Egyetem
Hidalgo, Elena
Spain, Barcelona
Universitat Pompeu Fabra Barcelona
Ariño, Joaquín
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Statistics
Citations: 15
Authors: 11
Affiliations: 7
Identifiers
Doi:
10.1111/mmi.12370
ISSN:
0950382X
e-ISSN:
13652958
Research Areas
Genetics And Genomics