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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Analysis of lysozyme‐specific immune responses by synthetic peptides. I. Characterization of antibody and T cell‐mediated responses to the N‐terminal peptide of hen egg‐white lysozyme
European Journal of Immunology, Volume 16, No. 1, Year 1986
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Description
The immunological reactivity against the N‐terminal region of hen egg‐white lysozyme (HEL) has been investigated by a synthetic peptide (PHEL) comprising residue 1–18 of HEL and by an analogue peptide (PREL) in which phenylalanine at position 3 is substituted by tyrosine. Both peptides are immunogenic in (C57BL/10 × DBA/2)F1 mice genetically responder to HEL. In C57BL/6 mice, genetically nonresponder to HEL, PREL induces anti‐peptide antibodies that also bind to PHEL whereas PHEL is not immunogenic. Thus, a single amino acid substitution in a synthetic peptide converts a nonresponder mouse strain into a responder one. Anti‐PHEL antibodies demonstrate a higher binding to HEL than anti‐PREL antibodies, indicating that phenylalanine at position 3 is important for induction of anti‐peptide antibodies able to recognize native HEL. At the T cell level the two peptides show very high bidirectional cross‐reactivity between themselves and with HEL for interleukin 2 production, antigen‐specific proliferation and delayed‐type hypersensitivity response, whereas conservation of phenylalanine at position 3 is required for induction of suppressor cells cross‐reactive with HEL. This indicates that the N‐terminal region of HEL contains epitope(s) able to induce the same level of helper T cell activity as the native HEL molecule. However, helper T cells do not discriminate between PHEL and PREL whereas phenylalanine at position 3 is critical for HEL‐specific suppressor T cell induction. Copyright © 1986 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Authors & Co-Authors
Sette, Alessandro D.
Italy, Rome
Ente Per le Nuove Tecnologie, L'energia e L'ambiente
Colizzi, Vittorio C.
Italy, Rome
Enea Centro Ricerche Casaccia
Appella, Ettore
United States, Bethesda
National Institutes of Health Nih
Doria, Gino
Italy, Rome
Ente Per le Nuove Tecnologie, L'energia e L'ambiente
Statistics
Citations: 15
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1002/eji.1830160102
ISSN:
00142980