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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
engineering
Efficacy of mesoporous silica nanoparticles in delivering BMP-2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells
Journal of Biomedical Materials Research - Part A, Volume 101 A, No. 6, Year 2013
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Description
We report the ability of aminated mesoporous silica nanoparticles (MSN-NH2) with large mesopore space and positive-charged surface to deliver genes within rat mesenchymal stem cells (MSCs). The amine functionalized inorganic nanoparticles were complexed with bone morphogenetic protein-2 (BMP2) plasmid DNA (pDNA) to study their transfection efficiency in MSCs. Intracellular uptake of the complex BMP2 pDNA/MSN-NH2 occurred significantly, with a transfection efficiency of approximately 68%. Furthermore, over 66% of the transfected cells produced BMP2 protein. The osteogenic differentiation of the transfected MSCs was demonstrated by the expression of bone-related genes and proteins including bone sialoprotein, osteopontin, and osteocalcin. The MSN-NH2 delivery vehicle for BMP2 pDNA developed in this study may be a potential gene delivery system for bone tissue regeneration. © 2012 Wiley Periodicals, Inc.
Authors & Co-Authors
Kim, T. H.
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
South Korea, Seoul
Dankook University
Kim, Meeju
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
South Korea, Seoul
Dankook University
Eltohamy, Mohamed R.
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
South Korea, Seoul
Dankook University
Egypt, Giza
National Research Centre
Yun, Ye Rang
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
South Korea, Seoul
Dankook University
Jang, Jun Hyeog
South Korea, Incheon
College of Medicine
Kim, Hae-won
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, College of Dentistry
Statistics
Citations: 59
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1002/jbm.a.34466
ISSN:
15493296
e-ISSN:
15524965
Research Areas
Cancer
Genetics And Genomics