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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Prevalence of Campylobacter species, Helicobacter pylori and Arcobacter species in stool samples from the Venda region, Limpopo, South Africa: Studies using molecular diagnostic methods
Journal of Infection, Volume 54, No. 6, Year 2007
Notification
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Description
Objectives: This study determined the prevalence of Campylobacter spp., Helicobacter pylori and Arcobacter spp. in stool samples from Venda in relation to diarrhea, intestinal inflammation and HIV status using specific molecular methods. Methods: Stool samples were collected from hospital patients (255) and primary school children (67). Genomic DNA was extracted from the stools and molecular methods including PCR, PCR followed by restriction analysis and multiplex PCR were used to test for the different organisms. The lactoferrin content of the stools was determined using commercial kits from TechLab (Blacksburg, VA, USA). Results: The prevalence of the different organisms was 50.6% for H. pylori, 10.2% for C. jejuni, 6.2% for A. butzleri, 6.5% for C. coli, 3.1% for C. concisus, 2.8% for A. cryaerophilus and 1.9% for A. skirrowii. Of all the organisms, only C. jejuni was significantly associated with diarrhea (84.8%) (χ2 = 21.025, P < 0.001) and elevated levels of lactoferrin (78.8%) (χ2 = 16.919, P < 0.005) and was an important pathogen associated with diarrhea among HIV positive individuals (22.8%). Conclusions: Campylobacter infections are common causes of gastroenteritis in Venda. Non-C. jejuni/coli Campylobacters such as C. concisus as well as A. butzleri and H. pylori may be involved in gastrointestinal diseases in the region but further studies are needed to confirm this hypothesis. © 2006 The British Infection Society.
Authors & Co-Authors
Samie, Amidou
South Africa, Thohoyandou
University of Venda
United States, Atlanta
Centers for Disease Control and Prevention
Obi, Chikwelu Larry
South Africa, Pretoria
School of Agriculture and Life Sciences
Barrett, Leah J.
United States, Atlanta
Centers for Disease Control and Prevention
Powell, S. M.
United States, Charlottesville
University of Virginia School of Medicine
Guerrant, Richard Littleton
United States, Atlanta
Centers for Disease Control and Prevention
Statistics
Citations: 160
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.jinf.2006.10.047
ISSN:
01634453
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study
Study Locations
South Africa