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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli
Journal of Antimicrobial Chemotherapy, Volume 57, No. 6, Year 2006
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Description
Objectives: To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods: A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515-18). Integrons were characterized by triplex real-time PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results: A gradient of resistance ranging from absence to high prevalence (resistance score of 18.7%) and a gradual increase in the prevalence of class 1 integrons (from 0% to 16%), both correlated with the increase in exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions: Our findings firmly establish that the current prevalence of antimicrobial resistance found in animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. © 2006 Oxford University Press.
Authors & Co-Authors
Skurnik, David
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Ruimy, Raymond
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Andremont, Antoine O.
France, Paris
Université Paris Cité
Amorin, Christine
France, Paris
Université Paris Cité
Rouquet, Pierre
Gabon, Franceville
Centre International de Recherches Medicales de Franceville
Picard, Bertrand
France, Paris
Université Paris Cité
Denamur, Erick
France, Paris
Université Paris Cité
Statistics
Citations: 221
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1093/jac/dkl122
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Study Design
Cross Sectional Study
Study Locations
Gabon