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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Oral fluid for the serological and molecular diagnosis of measles
International Journal of Infectious Diseases, Volume 14, No. 11, Year 2010
Notification
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Description
Objectives: Since measles presents mostly in children, a non-invasive sample collection technique such as oral fluid sampling would be very useful in the early detection of measles RNA and antibodies. The aim of this study was to validate the detection of anti-measles IgM and measles virus RNA in oral fluid and to make a comparison with the gold standard methods of ELISA using serum (Enzygnost® anti-Measles IgM) and in-house nested reverse transcriptase polymerase chain reaction (RT-PCR) using nasopharyngeal secretions. Methods: Three samples each from 73 measles-positive and 44 measles-negative subjects (serum, oral fluid, and nasopharyngeal secretions) were analyzed. Results: The anti-measles IgM ELISA (MicroImmune) on oral fluid was validated against the IgM ELISA (Siemens) for serum and this resulted in a sensitivity of 92% and specificity of 100%. A molecular nested RT-PCR using oral fluid was validated against the standard assay on nasopharyngeal secretions and gave a sensitivity of 100% and specificity of 100%. Conclusions: The results confirm that both serological and molecular oral fluid assays are suitable for routine use. The use of oral fluid samples for the detection of measles virus may encourage patients, general practitioners, and pediatricians to participate in the Belgian measles surveillance system and other epidemiological studies in the framework of the World Health Organization elimination program. © 2010 International Society for Infectious Diseases.
Authors & Co-Authors
Hutse, Veronik
Belgium, Brussels
Scientific Institute of Public Health, Brussels
van Hecke, K.
Belgium, Brussels
Scientific Institute of Public Health, Brussels
de Bruyn, R.
Belgium, Brussels
Scientific Institute of Public Health, Brussels
Samu, O.
Congo
National Laboratory for Measles
Lernout, Tinne
Belgium, Brussels
Scientific Institute of Public Health, Brussels
Muyembe-Tamfum, Jean Jacques
Congo
National Laboratory for Measles
Brochier, Bernard M.
Belgium, Brussels
Scientific Institute of Public Health, Brussels
Statistics
Citations: 38
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijid.2010.06.009
ISSN:
12019712
Research Areas
Health System And Policy
Infectious Diseases
Maternal And Child Health