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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Fertilization and embryonic development of human oocytes after cooling
Human Reproduction, Volume 7, No. 10, Year 1992
Notification
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Description
Injury to living cells resulting from rapid cooling to temperatures at or near 0°C has long been recognized, and the phenomenon, which is termed 'cold shock', has been known to occur in some mammalian gametes. Although human embryos have been successfully stored at low temperatures, cryopreservation of the human oocyte is proving to be more difficult. Whether or not this lack of success is a direct result of cellular injury brought about by 'cold shock' is the purpose of the current investigation. Human oocytes were cooled, in the absence of cryoprotectants, at two different cooling rates (-3°C/min and -1000+°C/min) to a temperature of 0°C and rewarmed prior to insemination. In both cases fertilization after cooling was similar to the rates achieved in a routine in-vitro fertilization and embryo transfer procedure. After cooling at -3°C/min, the rate of fertilization was 19/22 (86%) and after cooling at -1000+°C/min, 9/9 (100%), with non-cooled control rates of 62/87 (71%) and 35/50 (70%) respectively. Fertilized ooctyes from both groups were successfully cultured for a further 24 h before termination of the experiment. © 1992 Oxford University Press.
Authors & Co-Authors
Bernard, A.
United Kingdom, Cardiff
Cardiff University
Hunter, J. E.
United Kingdom, Cardiff
Cardiff University
United Kingdom, London
University Department of Obstetrics and Gynaecology
Fuller, Barry J.
United Kingdom, Cardiff
Cardiff University
United Kingdom, London
The Royal Free Hospital
Imoedemhe, Daniel A.G.
United Kingdom, Cardiff
Cardiff University
Saudi Arabia, Jeddah
Dr Soliman Fakeeh Hospital
Curtis, P.
United Kingdom, Cardiff
Cardiff University
United Kingdom, London
University Department of Obstetrics and Gynaecology
Jackson, A.
United Kingdom, Cardiff
Cardiff University
United Kingdom, London
University Department of Obstetrics and Gynaecology
Statistics
Citations: 22
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1093/oxfordjournals.humrep.a137592
ISSN:
02681161
Research Areas
Violence And Injury
Study Approach
Quantitative