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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Influence of growth regulators on callogenesis and somatic embryo development in date palm (Phoenix dactylifera L.) Sahelian cultivars
The Scientific World Journal, Volume 2012, Article 837395, Year 2012
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Description
This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The primary callogenesis from leaf explants of seedlings appeared highly dependent on genotype. Ahmar (80) and Amsekhsi (76) appeared highly callogenic, whereas Tijib (10) and Amaside (2) produced low amounts of calluses. 2,4-Dichlorophenoxyacetic acid appeared favorable to the induction of primary callogenesis and its effect was enhanced by the addition of benzyl adenine or adenine sulfate. Secondary friable calli obtained from chopped granular calli were used to initiate embryogenic cell suspensions in media supplied with 2,4-dichlorophenoxyacetic acid. Suspension cultures showed a growth rate of fourfold after four subcultures in presence of 2,4-dichlorophenoxyacetic acid 2mg/L. Our results showed that a seven-day transitory treatment with benzyl adenine 0,5mg/L was necessary to optimize embryos development. Naphthalene acetic acid induced the development of primary orthogravitropic roots during embryos germination. The comparison with cytofluorometry of nuclear DNA amounts showed no significant difference in ploidy level between regenerated plants and seedlings. Copyright © 2012 Djibril Sané et al.
Authors & Co-Authors
Sané, Djibril
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Aberlenc-Bertossi, Frédérique
France, Montpellier
Ird Centre de Montpellier
Diatta, Léopold Ibrahima Djitiningo
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Guéye, Badara
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Daher, Abdourahman
Djibouti, Djibouti
Centre D'etudes et de Recherche de Djibouti
Sagna, Maurice T.
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Duval, Yves
France, Montpellier
Ird Centre de Montpellier
Borg̀el, Alain
France, Montpellier
Ird Centre de Montpellier
Statistics
Citations: 30
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1100/2012/837395
e-ISSN:
1537744X
Research Areas
Cancer
Genetics And Genomics