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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Interrupted replication of hepatitis B virus in liver tissue of HBsAg carriers with hepatocellular carcinoma
Virology, Volume 166, No. 1, Year 1988
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Description
To search for events underlying reduction of peripheral viremia and integration of hepatitis B virus (HBV) DNA into the liver cell genome in long-term virus carriers with hepatocellular carcinoma, paired samples of liver and tumor tissue were analyzed by molecular hybridization and immunological methods. Most tumor tissues contained integrated viral DNA; in none was extrachromosomal HBV DNA detected. Integrated HBV DNA was also found in peritumor liver tissue in the majority of patients. However, liver of patients either with or without peripheral viremia also contained free HBV DNA and replicative intermediates. In three nonviremic patients with replicative HBV DNA in liver, viral core antigen expression was markedly reduced or absent, whereas viral envelope protein (surface antigen) expression was normal. In one case, replicative intermediates in liver were sensitive to DNase I digestion, indicating that viral DNA was not encapsidated in normal viral core particles. These results suggest that decreased or defective core antigen production can lead to reduced viremia associated with blocked virus assembly/secretion and accumulation of unencapsidated HBV DNA replicative intermediates in the liver cell. Accumulation of such HBV DNA molecular forms in the liver may lead to an increased propensity for HBV DNA to integrate into the host genome, which has been found with high frequency in hepatic neoplasms from patients infected with hepatitis B virus. © 1988.
Authors & Co-Authors
Raimondo, Giovanni
United States, New York
Marion Bessin Liver Research Center
Burk, Robert David
United States, New York
Marion Bessin Liver Research Center
Lieberman, Harvey M.
United States, New York
Marion Bessin Liver Research Center
Muschel, Joseph
United States, New York
Marion Bessin Liver Research Center
Hadziyannis, Stephanos J.
Greece, Athens
National and Kapodistrian University of Athens
Will, Hans
Germany, Planegg
Max-planck-institut Für Biochemie
Kew, Michael Charles
South Africa, Johannesburg
School of Clinical Medicine
Dusheiko, Geoffrey M.
South Africa, Johannesburg
School of Clinical Medicine
Shafritz, David A.
United States, New York
Marion Bessin Liver Research Center
United States, New York
Albert Einstein College of Medicine of Yeshiva University
Statistics
Citations: 72
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/0042-6822(88)90151-1
ISSN:
00426822
e-ISSN:
10960341
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases