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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Sequence analysis of a rainbow trout cDNA library and creation of a gene index
Cytogenetic and Genome Research, Volume 102, No. 1-4, Year 2003
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Description
Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5′ ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species. Copyright © 2003 S. Karger AG, Basel.
Authors & Co-Authors
Keele, John W.
United States, Washington, D.c.
Usda Agricultural Research Service
Brown, Gordon D.A.
Canada, Victoria
University of Victoria
Koop, Ben F.
Canada, Victoria
University of Victoria
Quackenbush, John F.
United States, Rockville
J. Craig Venter Institute
United States, Washington, D.c.
The George Washington University
Statistics
Citations: 95
Authors: 4
Affiliations: 5
Identifiers
Doi:
10.1159/000075773
ISSN:
14248581
Research Areas
Genetics And Genomics