Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Validation of an HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to analyze chlordecone in human serum
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 1229, Article 123894, Year 2023
Notification
URL copied to clipboard!
Description
Until 1993, chlordecone (CLD) was extensively used in banana fields in French West Indies. In a previous study, CLD was detected in 90 % of Martinican and Guadeloupean adult's serum. In order to simplify the analyses of CLD in the serum, a new QuEChERS-HPLC-MS/MS method was implemented and validated by the Pasteur Institute of Guadeloupe (IPG). This method was validated with accuracy profiles according to the French Standard NF V03-110 plus the ISO 15189 and European guidelines. Linearity, repeatability, accuracy, intermediate precision, specificity, limit of detection (LOD), limit of quantification (LOQ) and uncertainty were determined. The accuracy profile allowed the method to be validated between 0.06 µg L-1 and 1.00 µg L-1 of serum. The LOD was 0.02 µg L-1, the LOQ was 0.06 µg L-1 and the uncertainty of the method was 21 %. A comparison of 49 serum samples between the IPG (LC-MS/MS) and the LEAE-CART (GC-HRMS) laboratories demonstrated that this new method can reliably determine CLD in human serum. Stability tests were performed and duration of the storage of raw samples and extracts before analysis by HPLC-MS/MS. Raw samples were stable after collection for at least one week at 5 °C or 25 °C and for at least 3 months at −20 °C. Extracts in acetonitrile were stable for at least 1 month at −20 °C. These stability results facilitate the daily use of the method. This method should help the entire population of Guadeloupe and Martinique by allowing a routinely analyzed for CLD and will be useful for future projects aimed at improving population health monitoring. © 2023 The Author(s)
Authors & Co-Authors
Thomé, Jean Pierre
Belgium, Liege
Université de Liège
Scholl, Georges
Belgium, Liege
Université de Liège
Védy, Serge
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Ferdinand, Séverine
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Talarmin, Antoine
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Guyomard-Rabenirina, Stéphanie
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Statistics
Citations: 1
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.jchromb.2023.123894
ISSN:
15700232
Study Design
Cross Sectional Study