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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP)
Parasitology Research, Volume 109, No. 6, Year 2011
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Description
This study compared the prevalence of trypanosome infections estimated by PFR-loop-mediated isothermal amplification (LAMP) with conventional polymerase chain reaction (PCR) tests. One hundred forty eight cattle blood samples were collected from Robanda village, Mara region, Tanzania in April 2008. In conventional PCR, four sets of primers, specific for the detection of Trypanosoma sp., Trypanosoma brucei rhodesiense, Trypanosoma vivax, and Trypanozoon, as well as a modified LAMP were used. Conventional PCR detected no infection or up to 8, 1, and 3 infections with Trypanosoma congolense savannah, Trypanozoon, and T. vivax, respectively, whereas LAMP detected additional 44 Trypanozoon positive cases. Our results clearly indicate that the prevalence of Trypanozoon spp. in cattle in Robanda village estimated by PFR-LAMP (30.4%) was significantly higher than the estimates by PCR assays (0.6-2%). As such, future studies should target epidemiological surveys of Trypanozoon and T. brucei rhodesiense infections in possible reservoir animals by LAMP to further elucidate the actual prevalence of these parasites. © 2011 Springer-Verlag.
Authors & Co-Authors
Laohasinnarong, Dusit
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Thailand, Nakhon Pathom
Mahidol University
Thekisoe, Oriel M.M.
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
South Africa, Bloemfontein
University of the Free State
Malele, Imna I.
Tanzania, Tanga
Majani Mapana
Namangala, Boniface
Zambia, Lusaka
University of Zambia
Ishii, Akihiro
Japan, Sapporo
Hokkaido University
Goto, Yasuyuki
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Kawazu, Shinichiro
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Sugimoto, Chihiro
Japan, Sapporo
Hokkaido University
Inoue, Noboru I.
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Statistics
Citations: 42
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1007/s00436-011-2513-2
ISSN:
09320113
e-ISSN:
14321955
Study Design
Cross Sectional Study
Study Locations
Tanzania