Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Members of the uncultured bacterial candidate division WWE1 are implicated in anaerobic digestion of cellulose
MicrobiologyOpen, Volume 3, No. 2, Year 2014
Notification
URL copied to clipboard!
Description
Clones of the WWE1 (Waste Water of Evry 1) candidate division were retrieved during the exploration of the bacterial diversity of an anaerobic mesophilic (35 ± 0.5°C) digester. In order to investigate the metabolic function of WWE1 members, a 16S rRNA gene -based stable isotope probing (SIP) method was used. Eighty-seven percent of 16S r rRNA gene sequences affiliated to WWE1 candidate division were retrieved in a clone library obtained after polymerase chain reaction (PCR) amplification of enriched DNA fraction from anaerobic municipal solid waste samples incubated with 13C-cellulose, at the end of the incubation (day 63) using a Pla46F-1390R primer pair. The design of a specific WWE1 probe associated with the fluorescence in situ hybridization (FISH) technique corroborated the abundant representation of WWE1 members in our 13C-cellulose incubations. Secondary ion mass spectrometry-in situ hybridization (SIMSISH) using an iodine-labeled oligonucleotide probe combined with high-resolution nanometer-scale SIMS (NanoSIMS) observation confirmed the isotopic enrichment of members of WWE1 candidate division. The 13C apparent isotopic composition of hybridized WWE1 cells reached the value of about 40% early during the cellulose degradation process, suggesting that these bacteria play a role either in an extracellular cellulose hydrolysis process and/or in the uptake fermentation products. © 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
Authors & Co-Authors
Limam, Rim Driss
France, Antony
Ur Hban Hydrosystèmes et Bioprocédés
France, Gif-sur-yvette
Commissariat a L'energie Atomique et Aux Energies Alternatives
France, Evry
Université D'evry Val D'essonne
Tunisia, Sidi Thabet
Centre National Des Sciences et Technologies Nucleaires
Tunisia, Tunis
University of Carthage
Chouari, Rakia
Tunisia, Tunis
University of Carthage
Mazéas, Laurent
France, Antony
Ur Hban Hydrosystèmes et Bioprocédés
Wu, Ting Di
France, Paris
Inserm
France, Paris
Institut Curie
Li, Tianlun
France, Antony
Ur Hban Hydrosystèmes et Bioprocédés
Grossin-Debattista, Julien
France, Antony
Ur Hban Hydrosystèmes et Bioprocédés
Guerquin-Kern, Jean Luc
France, Paris
Inserm
France, Paris
Institut Curie
Saidi, Mouldi
Tunisia, Sidi Thabet
Centre National Des Sciences et Technologies Nucleaires
Landoulsi, Ahmed
Tunisia, Tunis
University of Carthage
Sghir, Abdelghani
France, Gif-sur-yvette
Commissariat a L'energie Atomique et Aux Energies Alternatives
France, Evry
Université D'evry Val D'essonne
Bouchez, T.
France, Antony
Ur Hban Hydrosystèmes et Bioprocédés
Statistics
Citations: 105
Authors: 11
Affiliations: 7
Identifiers
Doi:
10.1002/mbo3.144
e-ISSN:
20458827
Research Areas
Environmental
Genetics And Genomics
Noncommunicable Diseases