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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Visualization of grapevine root colonization by the Saharan soil isolate Saccharothrix algeriensis NRRL B-24137 using DOPE-FISH microscopy
Plant and Soil, Volume 370, No. 1-2, Year 2013
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Description
Background and aim: There is currently a gap of knowledge regarding whether some beneficial bacteria isolated from desert soils can colonize epi- and endophytically plants of temperate regions. In this study, the early steps of the colonization process of one of these bacteria, Saccharothrix algeriensis NRRL B-24137, was studied on grapevine roots to determine if this beneficial strain can colonize a non-natural host plant. An improved method of fluorescence in situ hybridization (FISH), the double labeling of oligonucleotide probes (DOPE)-FISH technique was used to visualize the colonization behavior of such bacteria as well as to determine if the method could be used to track microbes on and inside plants. Methods: A probe specific to Saccharothrix spp. was firstly designed. Visualization of the colonization behavior of S. algeriensis NRRL B-24137 on and inside roots of grapevine plants was then carried out with DOPE-FISH microscopy. Results: The results showed that 10 days after inoculation, the strain could colonize the root hair zone, root elongation zone, as well as root emergence sites by establishing different forms of bacterial structures as revealed by the DOPE-FISH technique. Further observations showed that the strain could be also endophytic inside the endorhiza of grapevine plants. Conclusions: Taking into account the natural niches of this beneficial strain, this study exemplifies that, in spite of its isolation from desert soil, the strain can establish populations as well as subpopulations on and inside grapevine plants and that the DOPE-FISH tool can allow to detect it. © 2013 Springer Science+Business Media Dordrecht.
Authors & Co-Authors
Compant, Stéphane
France, Toulouse
Institut National Polytechnique de Toulouse
Austria, Vienna
Austrian Institute of Technology
Muzammil, Saima
France, Toulouse
Institut National Polytechnique de Toulouse
Lebrihi, Ahmed
France, Toulouse
Institut National Polytechnique de Toulouse
Morocco, Meknes
Université Moulay Ismaïl
Mathieu, Florence
France, Toulouse
Institut National Polytechnique de Toulouse
Statistics
Citations: 4
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1007/s11104-013-1648-6
ISSN:
0032079X
Research Areas
Genetics And Genomics