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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Neurons are host cells for Mycobacterium tuberculosis
Infection and Immunity, Volume 82, No. 5, Year 2014
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Description
Mycobacterium tuberculosis infection of the central nervous system is thought to be initiated once the bacilli have breached the blood brain barrier and are phagocytosed, primarily by microglial cells. In this study, the interactions of M. tuberculosis with neurons in vitro and in vivo were investigated. The data obtained demonstrate that neurons can act as host cells for M. tuberculosis. M. tuberculosis bacilli were internalized by murine neuronal cultured cells in a time-dependent manner after exposure, with superior uptake by HT22 cells compared to Neuro-2a cells (17.7% versus 9.8%). Internalization of M. tuberculosis bacilli by human SK-N-SH cultured neurons suggested the clinical relevance of the findings. Moreover, primary murine hippocampusderived neuronal cultures could similarly internalize M. tuberculosis. Internalized M. tuberculosis bacilli represented a productive infection with retention of bacterial viability and replicative potential, increasing 2- to 4-fold within 48 h. M. tuberculosis bacillus infection of neurons was confirmed in vivo in the brains of C57BL/6 mice after intracerebral challenge. This study, therefore, demonstrates neurons as potential new target cells for M. tuberculosis within the central nervous system. © 2014, American Society for Microbiology.
Authors & Co-Authors
Randall, Philippa J.
South Africa, Cape Town
Faculty of Health Sciences
Hsu, Nai Jen
South Africa, Cape Town
Faculty of Health Sciences
Lang, Dirk M.
South Africa, Cape Town
Faculty of Health Sciences
Cooper, Susan
South Africa, Cape Town
Faculty of Health Sciences
Sebesho, Boipelo
South Africa, Cape Town
Faculty of Health Sciences
Allie, Nasiema
South Africa, Cape Town
Faculty of Health Sciences
Keeton, Roanne S.
South Africa, Cape Town
Faculty of Health Sciences
Francisco, Ngiambudulu M.
South Africa, Cape Town
Faculty of Health Sciences
Salie, Sumayah
South Africa, Cape Town
Faculty of Health Sciences
Labuschagné, Antoinette
South Africa, Cape Town
Faculty of Health Sciences
Quesniaux, Valérie F.J.
France, Orleans
Université D'orléans
Ryffel, Bernhard
France, Orleans
Université D'orléans
Kellaway, Lauriston A.
South Africa, Cape Town
Faculty of Health Sciences
Jacobs, Muazzam
South Africa, Cape Town
Faculty of Health Sciences
South Africa, Johannesburg
National Health Laboratory Service
Statistics
Citations: 19
Authors: 14
Affiliations: 3
Identifiers
Doi:
10.1128/IAI.00474-13
ISSN:
00199567
e-ISSN:
10985522