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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice: Implications for a subunit vaccine
Vaccine, Volume 25, No. 1, Year 2007
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Description
We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine. © 2006 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Mutwiri, George Kiremu
Canada, Saskatoon
University of Saskatchewan
See, Raymond H.
Canada, Vancouver
University of British Columbia, Faculty of Medicine
Brunham, Robert C.
Canada, Vancouver
University of British Columbia, Faculty of Medicine
Finlay, Barton Brett
Canada, Vancouver
The University of British Columbia
Kelvin, David J.
Canada, Toronto
University Health Network University of Toronto
Cochrane, Alan W.
Canada, Toronto
University of Toronto
Babiuk, Lorne A.
Canada, Saskatoon
University of Saskatchewan
Statistics
Citations: 70
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.vaccine.2006.06.084
ISSN:
0264410X
Research Areas
Covid