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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Bacillus Calmette-Guérin vaccination of human newborns induces T cells with complex cytokine and phenotypic profiles
Journal of Immunology, Volume 180, No. 5, Year 2008
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Description
The immune response to vaccination with bacillus Calmette-Guérin (BCG), the only tuberculosis vaccine available, has not been fully characterized. We used multiparameter flow cytometry to examine specific T cell cytokine production and phenotypic profiles in blood from 10-wk-old infants routinely vaccinated with BCG at birth. Ex vivo stimulation of whole blood with BCG for 12 h induced expression of predominantly IFN-γ, IL-2, and TNF-α in CD4+ T cells in seven distinct cytokine combinations. IL-4 and IL-10 expression was detected in CD4+ T cells at low frequencies and only in cells that did not coexpress type 1 cytokines. Specific CD8+ T cells were less frequent than CD4+ T cells and produced mainly IFN-γ and/or IL-2 and less TNF-α, IL-4, and IL-10. Importantly, many mycobacteria-specific CD4+ and CD8+ T cells did not produce IFN-γ. The predominant phenotype of BCG-specific type 1 T cells was that of effector cells, i.e., CD45RA-CCR7 -CD27+, which may reflect persistence of Mycobacterium bovis BCG in infants until 10 wk of age. Among five phenotypic patterns of CD4+ T cells, central memory cells were more likely to be IL-2 + and effector cells were more likely to be IFN-γ+. We concluded that neonatal vaccination with BCG induces T cells with a complex pattern of cytokine expression and phenotypes. Measuring IFN-γ production alone underestimates the magnitude and complexity of the host cytokine response to BCG vaccination and may not be an optimal readout in studies of BCG and novel tuberculosis vaccination. Copyright © 2008 by The American Association of Immunologists, Inc.
Authors & Co-Authors
Soares, Andreia P.
South Africa, Cape Town
University of Cape Town
Scriba, Thomas J.
South Africa, Cape Town
University of Cape Town
Joseph, Sarah
South Africa, Cape Town
University of Cape Town
Harbacheuski, Ryhor
United States, Newark
Public Health Research Institute
Murray, Rose Ann
United States, Newark
Public Health Research Institute
Gelderbloem, Sebastian J.
South Africa, Cape Town
University of Cape Town
Hawkridge, Anthony J.
South Africa, Cape Town
University of Cape Town
Hussey, Greg Dudley
South Africa, Cape Town
University of Cape Town
Maecker, Holden Terry
United States, San Jose
Bd Biosciences
Kaplan, Gilla
United States, Newark
Public Health Research Institute
Hanekom, Willem Albert
South Africa, Cape Town
University of Cape Town
South Africa, Cape Town
Faculty of Health Sciences
Statistics
Citations: 154
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.4049/jimmunol.180.5.3569
ISSN:
00221767
e-ISSN:
15506606
Research Areas
Maternal And Child Health