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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Agreement between commercially available ELISA and in-house Luminex SARS-CoV-2 antibody immunoassays
Scientific Reports, Volume 11, No. 1, Article 18984, Year 2021
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Description
Serological diagnostic of the severe respiratory distress syndrome coronavirus 2 (SARS-CoV-2) is a valuable tool for the determination of immunity and surveillance of exposure to the virus. In the context of an ongoing pandemic, it is essential to externally validate widely used tests to assure correct diagnostics and epidemiological estimations. We evaluated the performance of the COVID-19 ELISA IgG and the COVID-19 ELISA IgM/A (Vircell, S.L.) against a highly specific and sensitive in-house Luminex immunoassay in a set of samples from pregnant women and cord blood. The agreement between both assays was moderate to high for IgG but low for IgM/A. Considering seropositivity by either IgG and/or IgM/A, the technical performance of the ELISA was highly imbalanced, with 96% sensitivity at the expense of 22% specificity. As for the clinical performance, the negative predictive value reached 87% while the positive predictive value was 51%. Our results stress the need for highly specific and sensitive assays and external validation of diagnostic tests with different sets of samples to avoid the clinical, epidemiological and personal disturbances derived from serological misdiagnosis. © 2021, The Author(s).
Authors & Co-Authors
Santano, Rebeca
Spain, Barcelona
Instituto de Salud Global de Barcelona
Barrios, Diana
Spain, Barcelona
Instituto de Salud Global de Barcelona
Crispi, Fàtima
Spain, Barcelona
Institut D'investigacions Biomèdiques August pi I Sunyer - Idibaps
Crovetto, Francesca
Spain, Barcelona
Institut D'investigacions Biomèdiques August pi I Sunyer - Idibaps
Vidal, Marta
Spain, Barcelona
Instituto de Salud Global de Barcelona
Chi, Jordi
Spain, Barcelona
Instituto de Salud Global de Barcelona
Izquierdo, Luis
Spain, Barcelona
Instituto de Salud Global de Barcelona
Gratacós, Eduard
Spain, Barcelona
Institut D'investigacions Biomèdiques August pi I Sunyer - Idibaps
Moncunill, Gemma
Spain, Barcelona
Instituto de Salud Global de Barcelona
Dobaño, Carlota
Spain, Barcelona
Instituto de Salud Global de Barcelona
Statistics
Citations: 7
Authors: 10
Affiliations: 2
Identifiers
Doi:
10.1038/s41598-021-98296-y
ISSN:
20452322
Research Areas
Covid
Participants Gender
Female