Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evidence for Gag p24-specific CD4 T cells with reduced susceptibility to R5 HIV-1 infection in a UK cohort of HIV-exposed-seronegative subjects
AIDS, Volume 17, No. 16, Year 2003
Notification
URL copied to clipboard!
Description
Aim: To characterize HIV-1 Gag p24-specific CD4 cell responses in HIV-exposed-seronegative (ES) individuals. Methodology: Twelve ES individuals, of diverse ethnicity and wild type for the CCR5 Δ-32 mutation, were identified. Controls were HIV-negative blood donors. Gag p24-specific and total Vβ+ CD4 cells that expressed MIP-1β, IFN-γ and IL-2 were enumerated by intracytoplasmic cytokine staining. β-Chemokine expression was correlated with susceptibility to R5 HIV-1 infection, as measured by polymerase chain reaction for integrated HIV-1 and by p24 enzyme-linked immunosorbent assay. Results: Similar numbers of mitogen-stimulated and Vβ+ MIP-1β+, IFN-γ+ and IL-2+ T cells were found in ES and HIV-negative control subjects. However, all ES subjects tested had an HIV Gag p24-specific MIP-1β+, IFN-γ+ and IL-2+ CD4 T-cell response that was rare in controls. p24-Specific cells of all ES but no control subjects could be expanded by in-vitro Ag/IL-2 stimulation, and when re-stimulated with an overlapping peptide series showed evidence of a broad CD4 cell memory response directed against multiple regions of Gag p24. Mitogen-stimulated ES CD4 cells were as susceptible to HIV infection as those from control subjects, but p24-specific IFN-γ+ CD4 cells of six out of seven ES subjects tested were less susceptible to R5 HIV-1 infection than the counterpart fraction depleted of p24-specific IFN-γ+ cells. The addition of blocking anti-β-chemokine antibodies did not promote R5 HIV-1 infection of p24-specific IFN-γ+ cells. Conclusion: Specific CD4 cell immunity, characterized by a broadly directed memory Gag-p24 CD4 cell response and reduced susceptibility of specific CD4 cells to R5 HIV-1 infection, is a likely correlate of non-transmission. © 2003 Lippincott Williams & Wilkins.
Authors & Co-Authors
King, Deborah F.L.
United Kingdom, London
Faculty of Life Sciences & Medicine
Boaz, Mark J.
United Kingdom, London
Faculty of Life Sciences & Medicine
Sefia, Eseberuo
United Kingdom, London
Faculty of Life Sciences & Medicine
Easterbrook, Philippa Jane
United Kingdom, London
Faculty of Life Sciences & Medicine
Statistics
Citations: 19
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1097/00002030-200311070-00004
ISSN:
02699370
Research Areas
Cancer
Infectious Diseases
Study Design
Cohort Study
Study Locations
Multi-countries