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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus
PLoS ONE, Volume 8, No. 8, Article e71642, Year 2013
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Description
Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection. © 2013 Abd El Wahed et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3748043/bin/pone.0071642.s001.docx
Authors & Co-Authors
Abd El Wahed, Ahmed
Germany, Gottingen
Universitätsmedizin Göttingen
Egypt, Mansoura
Faculty of Veterinary Medicine
El-Deeb, Ayman Hany
Egypt, Giza
Faculty of Veterinary Medicine
El-Tholoth, Mohamed
Egypt, Mansoura
Faculty of Veterinary Medicine
Ahmed, Hanaa A.
Egypt, Giza
Animal Health Research Institute
Ahmed, Abeer
Egypt, Giza
Animal Health Research Institute
Hassan, Sayed
Egypt, Giza
Animal Health Research Institute
Hoffmann, Bernd
Germany, Tubingen
Friedrich-loeffler-institute
Haas, Bernd
Germany, Tubingen
Friedrich-loeffler-institute
Shalaby, Mohamed Abd El Hameed A.
Egypt, Giza
Faculty of Veterinary Medicine
Hufert, Frank T.
Germany, Gottingen
Universitätsmedizin Göttingen
Weidmann, Manfred W.
Germany, Gottingen
Universitätsmedizin Göttingen
Statistics
Citations: 194
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0071642
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Study Approach
Quantitative
Study Locations
Egypt