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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Enzyme-linked immunosorbent assay for diagnosis of acute sporadic hepatitis E in Egyptian children
The Lancet, Volume 339, No. 8789, Year 1992
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Description
Hepatitis E virus (H EV) is thought to be a cause of enterically transmitted non-A, non-B (ET-NANB) hepatitis. Waterborne epidemics have been recorded in many developing countries, mainly affecting young-to-middle-aged adults; sporadic infection and overt illness in children are rare. However, a convenient and sensitive diagnostic test for HEV infection is not yet available. We now report the use of a solid-phase enzyme-linked immunoassay (ELISA) that detects IgM and IgG antibody to HEV. In a prospective study of endemic acute hepatitis during 1986 in rural Benha, Egypt, 15 (42%) of 36 children with NANB hepatitis (from whom convalescent-phase sera were available every 3 months to 9 or 12 months) were positive for anti-HEV-IgG by ELISA. Of 20 sera from healthy Benha children (controls), 5 (25%) were also positive for anti-HEV-IgG. When evaluated for anti-HEV-IgM, 6 of the 15 IgG-positive children, but none of the controls, were IgM positive and were thus regarded as having confirmed acute HEV infections. These 6 cases together with 2 presumptive cases (IgM negative, IgG sero-conversion from positive to negative) presented sporadically over 9 months. This ELISA is a convenient method for the diagnosis of H EV infection; we have shown that the disease is present in Egypt, that it can occur endemically as sporadic cases, and that children do have overt infection. © 1992.
Authors & Co-Authors
Goldsmith, R. S.
United States, San Francisco
University of California, San Francisco
Yarbough, P. O.
United States, Redwood City
Genelabs Incorporated
Reyes, G. R.
United States, Redwood City
Genelabs Incorporated
Fry, K. E.
United States, Redwood City
Genelabs Incorporated
Gabor, K. A.
United States, Redwood City
Genelabs Incorporated
Kamel, Moamena A.
Egypt, Cairo
Faculty of Medicine
Amer, S.
Egypt, Giza
Cairo University
Zakaria, Soheir
Egypt, Benha
Faculty of Medicine
Gaffar, Y.
Egypt, Cairo
Ain Shams University
Statistics
Citations: 149
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1016/0140-6736(92)91647-Q
ISSN:
01406736
Research Areas
Infectious Diseases
Maternal And Child Health
Study Design
Cohort Study
Study Locations
Egypt