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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine
Systematic and Applied Microbiology, Volume 30, No. 2, Year 2007
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Description
A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O
2
, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366
T
(99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans. © 2006 Elsevier GmbH. All rights reserved.
Authors & Co-Authors
DeFlaun, Mary F.
United States, Atlanta
Geosyntec Consultants, Inc.
Fredrickson, James K.
United States, Richland
Pacific Northwest National Laboratory
Dong, H.
United States, Oxford
Miami University
Pfiffner, Susan M.
United States, Oak Ridge
University of Tennessee
Onstott, Tullis C.
United States, Princeton
Princeton University
Balkwill, David L.
United States, Tallahassee
Florida State University
Streger, S. H.
United States, Lawrenceville
Shaw Environmental, Inc.
Stackebrandt, Erko
Unknown Affiliation
Knoessen, S.
South Africa, Bloemfontein
University of the Free State
van Heerden, Esta
South Africa, Bloemfontein
University of the Free State
Statistics
Citations: 55
Authors: 10
Affiliations: 8
Identifiers
Doi:
10.1016/j.syapm.2006.04.003
e-ISSN:
16180984
Research Areas
Environmental
Study Locations
South Africa