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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Field evaluation of the Abbott ARCHITECT HIV Ag/Ab Combo immunoassay
Journal of Clinical Virology, Volume 58, No. SUPPL1, Year 2013
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Description
Background: Fourth generation assays for HIV diagnosis are progressively being introduced into routine services, due to their improvement of diagnosis. In spite of this, HIV diagnosis remains a challenge in sub-Saharan Africa, due to false positive reactivity. There is a continuous need for field evaluations and routine validations of fourth generation HIV tests in African populations. Objectives: Evaluate the performances of the ARCHITECT HIV Ag/Ab kit (Abbott) in a population living in an African setting-Cameroon compared to a population living in a European setting-France. Study design: 645 HIV samples from both France and Cameroon were evaluated. The positive panel (378 samples) included a diverse series of HIV-1 variants (groups M, N, O, and P) as well as HIV-2 samples. Results were compared to original diagnosis done with other 4th generation assays (AxSYM HIV Ag/Ab (Abbott) and Vidas HIV DUO QUICK) (bioMérieux). Results: Sensitivity of the ARCHITECT was 100% in both sites. It diagnosed all variants of the panel with different reactivity profiles following strain diversity. A wider range of reactivity was observed for group O. Specificity was slightly lower (97.6%) in Cameroon than in France (98.6%), probably due to a higher rate of false positive reactivity. ARCHITECT HIV Ag/Ab assay had high performances in clinical sensitivity and specificity and is adapted to the wide genetic diversity of viruses circulating in West Central Africa. Conclusion: Our results further highlight the need to evaluate HIV diagnostic tests before introduction into routine diagnostic services both in the North and in the South. © 2013 Elsevier B.V.
Authors & Co-Authors
Kfutwah, Anfumbom Kitu Womeyi
Cameroon, Yaounde
Centre Pasteur du Cameroun
Lémée, Véronique
France, Rouen
Hopital Charles Nicolle
Ngono, Hélène Valérie
Cameroon, Yaounde
Centre Pasteur du Cameroun
de Oliveira, Fabienne
France, Rouen
Hopital Charles Nicolle
Njouom, Richard
Cameroon, Yaounde
Centre Pasteur du Cameroun
Plantier, Jean Christophe
France, Rouen
Hopital Charles Nicolle
Statistics
Citations: 20
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.jcv.2013.08.015
ISSN:
13866532
e-ISSN:
18735967
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Cameroon