Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Deep proteome mapping of mouse kidney based on OFFGel prefractionation reveals remarkable protein post-translational modifications
Journal of Proteome Research, Volume 13, No. 3, Year 2014
Notification
URL copied to clipboard!
Description
Performing a comprehensive nonbiased proteome analysis is an extraordinary challenge due to sample complexity and wide dynamic range, especially in eukaryotic tissues. Thus, prefractionation steps conducted prior to mass spectrometric analysis are critically important to reduce complex biological matrices and allow in-depth analysis. Here we demonstrated the use of OFFGel prefractionation to identify more low abundant and hydrophobic proteins than in a nonfractionated sample. Moreover, OFFGel prefractionation of a kidney protein sample was able to unveil protein functional relevance by detecting PTMs, especially when prefractionation was augmented with a targeted enrichment strategy such as TiO2 phospho-enrichment. The OFFGel-TiO2 combination used in this study was comparable to other global phosphoproteomics approaches (SCX-TiO2, ERLIC-TiO2, or HILIC-TiO 2). The detailed mouse kidney proteome with the phosphopeptide enrichment presented here serves as a useful platform for a better understanding of how the renal protein modification machinery works and, ultimately, will contribute to our understanding of pathological processes as well as normal physiological renal functions. © 2014 American Chemical Society.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3993965/bin/pr401122m_si_001.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3993965/bin/pr401122m_si_002.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3993965/bin/pr401122m_si_003.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC3993965/bin/pr401122m_si_004.xlsx
Authors & Co-Authors
Magdeldin, Sameh
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Egypt, Ismailia
Faculty of Veterinary Medicine
Yamamoto, Keiko
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Yoshida, Yutaka
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Xu, Bo
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Zhang, Ying
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Fujinaka, Hidehiko
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Yaoita, Eishin
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Yates, John R.R.
United States, San Diego
Scripps Research Institute
Yamamoto, Tadashi
Japan, Niigata
Niigata University, Graduate School of Medical and Dental Science
Statistics
Citations: 21
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1021/pr401122m
ISSN:
15353893
e-ISSN:
15353907
Study Design
Case Study
Study Approach
Qualitative