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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres
Journal of Clinical Microbiology, Volume 47, No. 12, Year 2009
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Description
Bacterial vaginosis (BV) is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease. In contrast to the Lactobacillus-dominated normal vaginal microbiota, BV is characterized by a lack of lactobacilli and an abundance of anaerobic and gram-negative organisms, including Gardnerella vaginalis and Atopobium vaginae. To date, the laboratory diagnosis of BV has relied upon the fulfillment of criteria determined by microscopic observation of Gram-stained vaginal swabs. We describe a molecular-based method for the easy determination of the species profile within the vaginal microbiota based on the amplification of the chaperonin-60 genes of all bacteria present in the swab and hybridization of the amplicon to species-specific oligonucleotide-coupled fluorescent beads that are identified by flow cytometry with a Luminex instrument. We designed a nineplex Luminex array for characterization of the vaginal microbiota and applied it to the analysis of vaginal swabs from individuals from Africa and North America. Using the presence of A. vaginae or G. vaginalis, or both, as the defining criterion for BV, we found that the method was highly specific and sensitive for the diagnosis of BV using microscopy as a gold standard. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2786665/bin/supp_47_12_4067__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC2786665/bin/supp_47_12_4067__Figure_6_supplemental.xls
Authors & Co-Authors
Dumonceaux, Tim J.
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Schellenberg, John J.
Canada, Winnipeg
University of Manitoba
Goleski, Vanessa
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Hill, Janet E.
Canada, Saskatoon
University of Saskatchewan
Canada, Vancouver
Provincial Health Services Authority
Jaoko, Walter G.
Kenya, Nairobi
University of Nairobi
Kimani, Joshua
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Kenya, Nairobi
University of Nairobi
Money, Deborah M.
Canada, Vancouver
The University of British Columbia
Canada, Vancouver
Provincial Health Services Authority
Ball, Terry Blake
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Canada, Winnipeg
University of Manitoba
Canada, Ottawa
Public Health Agency of Canada
Plummer, Francis Allan
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Canada, Winnipeg
University of Manitoba
Severini, Alberto
Canada, Winnipeg
Canadian Science Centre for Human and Animal Health
Canada, Winnipeg
University of Manitoba
Statistics
Citations: 63
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1128/JCM.00112-09
ISSN:
00951137
Research Areas
Genetics And Genomics