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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment
Food Chemistry, Volume 348, Article 129110, Year 2021
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Description
Insects have been consumed by people for millennia and have recently been proposed as a complementary, sustainable source of protein to feed the world's growing population. Insects and crustaceans both belong to the arthropod family. Crustacean (shellfish) allergies are common and potentially severe; hence, the cross-reactivity of the immune system with insect proteins is a potential health concern. Herein, LC-MS/MS was used to explore the proteome of whole, roasted whole and roasted powdered cricket products. Eight protein extraction protocols were compared using the total number of protein and distinct peptide identifications. Within these data, 20 putative allergens were identified, of which three were arginine kinase (AK) proteoforms. Subsequently, a multiple reaction monitoring MS assay was developed for the AK proteoforms and applied to a subset of extracts. This targeted assay demonstrated that allergen abundance/detectability varies according to the extraction method as well as the food processing method. © 2021 Elsevier Ltd
Authors & Co-Authors
Juhász, Angéla
Australia, Perth
Edith Cowan University
Byrne, Keren A.
Australia, Canberra
Commonwealth Scientific and Industrial Research Organisation
Maurer-Stroh, Sebastian
Singapore, Singapore City
Agency for Science, Technology and Research, Singapore
Singapore, Singapore City
National University of Singapore
Lopata, Andreas Ludwig
Australia, Townsville
James Cook University
Statistics
Citations: 27
Authors: 4
Affiliations: 5
Identifiers
Doi:
10.1016/j.foodchem.2021.129110
ISSN:
03088146
Research Areas
Food Security
Study Design
Cross Sectional Study