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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Multilocus microsatellite genotyping array for investigation of genetic epidemiology of Pneumocystis jirovecii
Journal of Clinical Microbiology, Volume 52, No. 5, Year 2014
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Description
Pneumocystis jirovecii is a symbiotic respiratory fungus that causes pneumonia (PcP) in immunosuppressed patients. Because P. jirovecii cannot be reliably cultured in vitro, it has proven difficult to study and gaps in our understanding of the organism persist. The release of a draft genome for the organism opens the door for the development of new genotyping approaches for studying its molecular epidemiology and global population structure. We identified and validated 8 putatively neutral microsatellite markers and 1 microsatellite marker linked to the dihydropteroate synthase gene (dhps), the enzymatic target of sulfa drugs used for PcP prevention and treatment. Using these tools, we analyzed P. jirovecii isolates from HIV-infected patients from three geographically distant populations: Uganda, the United States, and Spain. Among the 8 neutral markers, we observed high levels of allelic heterozygosity (average He, 0.586 to 0.842). Consistent with past reports, we observed limited global population structuring, with only the Ugandan isolates showing minor differentiation from the other two populations. In Ugandan isolates that harbored mutations in dhps, the microsatellite locus linked to dhps demonstrated a depressed He, consistent with positive directional selection for sulfa resistance mutations. Using a subset of these microsatellites, analyses of individual and paired samples from infections in San Francisco, CA, showed reliable typeability within a single infection and high discriminatory power between infections. These features suggest that this novel microsatellite typing approach will be an effective tool for molecularepidemiological investigations into P. jirovecii population structure, transmission, and drug resistance. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3993678/bin/supp_52_5_1391__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3993678/bin/JCM.02531-13_zjm999093297so1.pdf
Authors & Co-Authors
Parobek, Christian M.
United States, Chapel Hill
Unc School of Medicine
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Jiang, Linda Y.
United States, Cleveland
Case Western Reserve University
Patel, Jaymin C.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Álvarez-Martínez, Míriam José
Spain, Barcelona
Instituto de Salud Global de Barcelona
Miró, José María
Spain, Barcelona
Institut D'investigacions Biomèdiques August pi I Sunyer - Idibaps
Worodria, William O.
Uganda, Kampala
Makerere University
Andama, Alfred Onubia
Uganda, Kampala
Makerere University
Fong, Serena
United States, San Francisco
University of California, San Francisco
Huang, Laurence
United States, San Francisco
University of California, San Francisco
United States, Maywood
Division of Pulmonary and Critical Care Medicine
Meshnick, Steven Richard
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Taylor, Steve M.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
United States, Durham
Duke University Medical Center
Juliano, Jonathan J.
United States, Chapel Hill
Unc School of Medicine
Statistics
Citations: 27
Authors: 12
Affiliations: 9
Identifiers
Doi:
10.1128/JCM.02531-13
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Uganda