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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII
Biological Chemistry, Volume 387, No. 5, Year 2006
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Description
Extracellular matrix and soluble plasma proteins generate peptides that regulate biological activities such as cell growth, differentiation and migration. Bradykinin, a peptide released from kininogen by kallikreins, stimulates vasodilatation and endothelial cell proliferation. Various classes of substances can potentiate these biological actions of bradykinin. Among them, the best studied are bradykinin potentiating peptides (BPPs) derived from snake venom, which can also strongly inhibit angiotensin I-converting enzyme (ACE) activity. We identified and synthesized sequences resembling BPPs in the vicinity of potential proteolytic cleavage sites in the collagen XVIII molecule, close to endostatin. These peptides were screened as inhibitors of human recombinant wild-type ACE containing two intact functional domains; two full-length ACE mutants containing only a functional C- or N-domain catalytic site; and human testicular ACE, a natural form of the enzyme that only contains the C-domain. The BPP-like peptides inhibited ACE in the micromolar range and interacted preferentially with the C-domain. The proteolytic activity involved in the release of BPP-like peptides was studied in human serum and human umbilical-vein endothelial cells. The presence of enzymes able to release these peptides in blood led us to speculate on a physiological mechanism for the control of ACE activities. Copyright © by Walter de Gruyter.
Authors & Co-Authors
Farias, Shirley L.
Brazil, Sao Paulo
Universidade Federal de São Paulo
Sabatini, Regiane A.
Brazil, Sao Paulo
Universidade Federal de São Paulo
Sampaio, Tatiana C.
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
Hirata, Izaura Yoshico
Brazil, Sao Paulo
Universidade Federal de São Paulo
Cezari, Maria Helena S.
Brazil, Sao Paulo
Universidade Federal de São Paulo
Juliano, Maria A.
Brazil, Sao Paulo
Universidade Federal de São Paulo
Sturrock, Edward D.
South Africa, Cape Town
Faculty of Health Sciences
Carmona, Adriana Karaoglanovic
Brazil, Sao Paulo
Universidade Federal de São Paulo
Juliano, Luiz
Brazil, Sao Paulo
Universidade Federal de São Paulo
Statistics
Citations: 13
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1515/BC.2006.078
e-ISSN:
14374315