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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Interleukin-4 induced by measles virus and measles-derived peptides as measured by IL-4 receptor-blocking ELISA
Journal of Immunological Methods, Volume 287, No. 1-2, Year 2004
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Description
Interleukin-4 (IL-4) is a signature cytokine for T-helper 2 (Th2) type immune responses in humans. However, data on antigen-specific secretion of IL-4 is limited due to difficulties detecting IL-4. We evaluated an IL-4 receptor-blocking assay for the detection of secreted IL-4 in peripheral blood mononuclear cells (PBMC) stimulated in vitro with measles virus (MV) and MV-derived nucleoprotein (N) and phosphoprotein (P) peptides. We recruited 20 healthy subjects, previously immunized with two doses of measles-mumps-rubella- II (MMR-II) vaccine. We evaluated the cellular and humoral immune status of these study subjects by an in vitro lymphoproliferation assay and an enzyme-linked immunosorbent assay (ELISA), respectively. We analyzed the MV-induced and N and P peptide-induced IL-4 levels in PBMC culture supernatants. Using the IL-4 receptor blocking assay, 50% of the subjects were positive for secreted IL-4 in response to MV stimulation, and 5% and 23.1% of study subjects were positive for secreted IL-4 in response to MV-derived N and P peptides, respectively. In contrast, we did not find any positive secreted IL-4 response to MV using conventional ELISA without IL-4 receptor-blocking antibody in our optimization study. Further, we found very low frequencies of IL-4 secreting cells using an alternate ELISpot technique, accounting for only a 5% positive response to MV and no response to P peptide. We propose that the IL-4 receptor-blocking assay is an easy-to-adapt technique for screening antigen-specific immune responses in large-scale population-based studies. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Howe, Rawleigh Craig
Unknown Affiliation
Jacobson, Robert M.
United States, Rochester
Mayo Clinic
Poland, Gregory A.
United States, Rochester
Mayo Clinic
Statistics
Citations: 24
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/j.jim.2004.02.002
ISSN:
00221759
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study